TITLE

Study of potential xanthine oxidase inhibitors: In silico and in vitro biological activity

AUTHOR(S)
Umamaheswari, Muthuswamy; Madeswaran, Arumugam; Asokkvunar, Kuppusamy; Sivashanmugam, Thirumalaisamy; Subhadradevi, Varadharajan; Jagannath, Puliyath
PUB. DATE
December 2011
SOURCE
Bangladesh Journal of Pharmacology;Dec2011, Vol. 6 Issue 2, p117
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
In an attempt to develop potent anti gout agents, coumarin derivatives and polyphenolic compounds were selected for present study. The docking energy of 2-benzyl coumarin was found to be -7.50 kcal/mol which was less than that of the standard allopurinol (-4.47 kcal/mol). All the selected compounds were found to exhibit lower binding energy (-7.50 to -4.68 kcal/mol) than allopurinol. Docking results confirm that selected compounds showed greater inhibition of xanthine oxidase due to their active binding sites. In xanthine oxidase assay, IC50 value of 2-benzyl coumarin was found to be 26 ± 1.16 ng/ mL, whereas that of allopurinol was 24 ± 0.28 ng/mL. All the compounds exhibited IC50 values ranging between 26 ± 1.16 to 58 ± 0.74 ng/mL. In enzyme kinetic studies, coumarin derivatives showed competitive and polyphenolic compounds showed non competitive type of enzyme inhibition. It can be concluded that coumarin derivatives could be a remedy for the treatment of gout and related inflammatory disorders.
ACCESSION #
75498892

 

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