IL-12 concentrations in the aqueous humor and serum of diabetic retinopathy patients

Gverović Antunica, Antonela; Karaman, Ksenija; Znaor, Ljubo; Sapunar, Ada; Buško, Vesna; Puzović, Velibor
June 2012
Graefe's Archive of Clinical & Experimental Ophthalmology;Jun2012, Vol. 250 Issue 6, p815
Academic Journal
Background: Previous studies suggest that inflammation plays an important part in the pathogenesis of diabetes. Cytokines may have a role in both positive and negative control of immunological reactions. Among many cytokines, interleukin 12 (IL-12) is known to be a strong pro-inflammatory cytokine. Methods: A total of 76 participants were enrolled in this study and classified into four groups: 23 diabetic patients with non-treated retinopathy, 17 diabetic patients with treated retinopathy, 12 diabetic patients without retinopathy, and 24 healthy control patients. Serum and aqueous humor samples were taken for the analysis of IL-12 concentration. Results: The aqueous humor IL-12 concentration was significantly elevated in the patients with non-treated diabetic retinopathy (χ(3, n = 76) = 27.137; p < 0.001). No significant differences in IL-12 serum concentrations between the groups were found ( F = 0.405, p = 0.750). Correlation analysis of IL-12 concentrations in the serum and aqueous humor showed a linear association between the two variables only in non-diabetic patients ( p = 0.003). Conclusions: This is the first study to show a significantly higher concentration of pro-inflammatory cytokine IL-12 in the aqueous humor of non-treated diabetic retinopathy patients in comparison with diabetic patients treated for retinopathy, without retinopathy, or with healthy individuals. Because the serum levels of IL-12 did not differ considerably between the studied groups, it is plausible that this is due to its local production and secretion.


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