Identification and enumeration of Microcystis using a sandwich hybridization assay

Zhu, Jing; Li, Xian; Du, Shi
April 2012
Journal of Microbiology;Apr2012, Vol. 50 Issue 2, p186
Academic Journal
No abstract available.


Related Articles

  • Infectious Diseases: Diagnosis Utilizing DNA Probes. McGowan, Karin L. // Clinical Pediatrics;Apr1989, Vol. 28 Issue 4, p157 

    The article focuses on the application of nucleic acid probes and hybridization techniques in the diagnosis of infectious diseases. The steps in constructing a nucleic acid probe are the following: first, identification of a sequence of DNA that is unique to a particular organism, next, using...

  • Detection of microcystin-producing in Guanqiao Lake using a sandwich hybridization assay. Zhu, Jing Ping; Du, Shi; Li, Xian // Canadian Journal of Microbiology;Apr2012, Vol. 58 Issue 4, p442 

    Based on sequence analyses of the mcyJ gene from strains, a probe pair TJF and TJR was designed and a sandwich hybridization assay (SHA) was established to quantitatively detect microcystin-producing Microcystis. Through BLAST and cyanobacterial culture tests, TJF and TJR were demonstrated to be...

  • Molecular Detection of Marine Invertebrate Larvae. Goffredi, Shana K.; Jones, William J.; Scholin, Christopher A.; Marin III, Roman; Vrijenhoek, Robert C. // Marine Biotechnology;Mar/Apr2006, Vol. 8 Issue 2, p149 

    The ecological patterns of many invertebrate larvae remain an ongoing mystery, in large part owing to the difficult task of detecting them in the water column. The development of nucleic-acid–based technology has the potential to resolve this issue by direct identification and monitoring...

  • FISH assay may be useful monitor of TCC recurrence. Guttman, Cheryl // Urology Times;Dec2002, Vol. 30 Issue 12, p7 

    Investigates the use of fluorescence in situ hybridization (FISH) assay for monitoring recurrence of bladder cancer. Identification of the molecular tumor markers by diagnostic test; Causes of the cancer relapse; Classification of tumor by FISH assay.

  • Molecular probe sets for the detection of toxic algae for use in sandwich hybridization formats. Sonja Diercks; Katja Metfies; Linda K. Medlin // Journal of Plankton Research;Apr2008, Vol. 30 Issue 4, p439 

    Molecular probes can be used for early and rapid detection of toxic algal species. The sandwich hybridization requires two probes for each species, a capture probe and a nearly adjacent signal probe. Probe sets for the species-specific identification of the toxic algal species Gymnodinium...

  • A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples. Kumanan, Vijayarani; Nugen, Sam R.; Baeumner, Antje J.; Yung-Fu Chang // Journal of Veterinary Science;2009, Vol. 10 Issue 1, p35 

    A simple, membrane-strip-based lateral-flow (LF) biosensor assay and a high-throughput microtiter plate assay have been combined with a reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of a small number (ten) of viable Mycobacterium (M.) avium subsp. paratuberculosis...

  • Detection of the dinoflagellate, Cochlodinium polykrikoides, that forms algal blooms using sandwich hybridization integrated with nuclease protection assay. Suh, Sung-Suk; Park, Mirye; Hwang, Jinik; Kil, Eui-Joon; Lee, Sukchan; Lee, Taek-Kyun // Biotechnology Letters;Jan2016, Vol. 38 Issue 1, p57 

    Objectives: To detect Cochlodinum polykrikoides in long-term monitoring and high-throughput sampling projects using an integrated sandwich hybridization and nuclease protection assay (NPA-SH). Results: The specificity of the probes was verified with individual and mixed cultures as well as field...

  • DNA Biosensor for Microcystis spp. Sequence Detection by Using Methylene Blue and Ruthenium Complex as Electrochemical Hybridization Labels. Erdem, Arzum; Kerman, Kagan; Meric, Burcu; Ozkan, Dilsat; Kara, Pinar; Ozsoz, Mehmet // Turkish Journal of Chemistry;2002, Vol. 26 Issue 6, p851 

    Presents a study that examined an electrochemical biosensor for the voltammetric detection of DNA sequences related to the bloomforming genera of Microcystis. Background on the uses of DNA biosensor technologies; Functions of microcystin polypeptides; Analysis of the hybrids formed on the...

  • The quantitative real-time PCR applications in the monitoring of marine harmful algal bloom (HAB) species. Antonella, Penna; Luca, Galluzzi // Environmental Science & Pollution Research;Oct2013, Vol. 20 Issue 10, p6851 

    In the last decade, various molecular methods (e.g., fluorescent hybridization assay, sandwich hybridization assay, automatized biosensor detection, real-time PCR assay) have been developed and implemented for accurate and specific identification and estimation of marine toxic microalgal...

  • Effect of Ozonation on the Removal of Cyanobacterial Toxins during Drinking Water Treatment. Hoeger, Stefan J.; Dietrich, Daniel R.; Hitzfeld, Bettina C. // Environmental Health Perspectives;Nov2002, Vol. 110 Issue 11, p1127 

    Investigates the efficacy of ozonation combined with various filtration steps under different cyanobacterial bloom conditions. Presence of cyanobacterial toxins determined using the protein phosphatase inhibition assay; Microcystis aeruginosa; Planktothrix rubescens.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics