TITLE

Leishmania (Viannia) panamensis expresses a nuclease with molecular and biochemical features similar to the Endonuclease G of higher eukaryotes

AUTHOR(S)
Toro-Londo�o, Miguel A.; Pati�o, Edwin Bairon; Robledo, Sara Mar�a; Jim�nez-Ruiz, Antonio; Alzate, Juan Fernando
PUB. DATE
October 2011
SOURCE
Colombia M�dica;oct-dec2011, Vol. 42 Issue 4, p154
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Objective: To characterize the molecular and biochemical features of the Endonuclease G of Leishmania (Viannia) panamensis. Methods: The gene of the putative L. (V.) panamensis Endonuclease G was amplified, cloned, and sequenced. The recombinant protein was produced in a heterologous expression system and biochemical assays were run to determine its ion, temperature, and pH preferences. Results: The L. (V.) panamensis rENDOG has biochemical features similar to those found in other trypanosomatids and higher eukaryotes. In addition, phylogenetic analysis revealed a possible evolutionary relationship with metazoan ENDOG. Conclusions: L. (V.) panamensis has a gene that codifies an ENDOG homologous to those of higher organisms. This enzyme can be produced in Escherichia coli and is able to degrade covalently closed circular double-stranded DNA. It has a magnesium preference, can be inhibited by potassium, and is able to function within a wide temperature and pH range.
ACCESSION #
74588354

 

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