TITLE

Phosphorylcholine Allows for Evasion of Bactericidal Antibody by Haemophilus influenzae

AUTHOR(S)
Clark, Sarah E.; Snow, Julian; Jianjun Li; Zola, Tracey A.; Weiser, Jeffrey N.
PUB. DATE
March 2012
SOURCE
PLoS Pathogens;Mar2012, Vol. 8 Issue 3, Special section p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The human pathogen Haemophilus influenzae has the ability to quickly adapt to different host environments through phase variation of multiple structures on its lipooligosaccharide (LPS), including phosphorylcholine (ChoP). During colonization with H. influenzae, there is a selection for ChoP+ phase variants. In a murine model of nasopharyngeal colonization, this selection is lost in the absence of adaptive immunity. Based on previous data highlighting the importance of natural antibody in limiting H. influenzae colonization, the effect of ChoP expression on antibody binding and its bactericidal activity was investigated. Flow cytometric analysis revealed that ChoP+ phase variants had decreased binding of antibody to LPS epitopes compared to ChoP2 phase variants. This difference in antibody binding correlated with increased survival of ChoP+ phase variants in the presence of antibody-dependent, complement-mediated killing. ChoP+ phase variants were also more resistant to trypsin digestion, suggesting a general effect on the physical properties of the outer membrane. Moreover, ChoP-mediated protection against antibody binding correlated with increased resilience of outer membrane integrity. Collectively, these data suggest that ChoP expression provides a selective advantage during colonization through ChoP-mediated effects on the accessibility of bactericidal antibody to the cell surface.
ACCESSION #
74386380

 

Related Articles

  • Effects of Vaccination with 10-Valent Pneumococcal Non-Typeable Haemophilus influenza Protein D Conjugate Vaccine (PHiD-CV) on the Nasopharyngeal Microbiome of Kenyan Toddlers. Feazel, Leah M.; Santorico, Stephanie A.; Robertson, Charles E.; Bashraheil, Mahfudh; Scott, J. Anthony G.; Frank, Daniel N.; Hammitt, Laura L. // PLoS ONE;Jun2015, Vol. 10 Issue 6, p1 

    Objective: Pneumococcal conjugate vaccines reduce the prevalence of vaccine serotypes carried in the nasopharynx. Because this could alter carriage of other potential pathogens, we assessed the nasopharyngeal microbiome of children who had been vaccinated with 10-valent pneumococcal...

  • Bacterial pathogenesis: Nosy neighbours. Amoils, Shannon // Nature Reviews Microbiology;Oct2005, Vol. 3 Issue 10, p747 

    The article reports on the study about the competitive interaction of Streptococcus pneumoniae and Haemophilus influenza (H. influenzae) that commonly reside in human nasopharynx. The researchers simultaneously introduced the two bacteria into the nasopharynx of mice and found that both bacteria...

  • Increased Nasopharyngeal Density and Concurrent Carriage of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis Are Associated with Pneumonia in Febrile Children. Chochua, Sopio; D'Acremont, Valérie; Hanke, Christiane; Alfa, David; Shak, Joshua; Kilowoko, Mary; Kyungu, Esther; Kaiser, Laurent; Genton, Blaise; Klugman, Keith P.; Vidal, Jorge E. // PLoS ONE;12/1/2016, Vol. 11 Issue 12, p1 

    Background: We assessed nasopharyngeal (NP) carriage of five pathogens in febrile children with and without acute respiratory infection (ARI) of the upper (URTI) or lower tract, attending health facilities in Tanzania. Methods: NP swabs collected from children (N = 960) aged 2 months to 10...

  • Development of a serological assay to predict antibody bactericidal activity against non-typeable Haemophilus influenzae. Ercoli, Giuseppe; Baddal, Buket; Alessandra, Greco; Marchi, Sara; Petracca, Roberto; Aricò, Beatrice; Pizza, Mariagrazia; Soriani, Marco; Rossi-Paccani, Silvia // BMC Microbiology;2015, Vol. 15 Issue 1, p1 

    Background: Non-typeable Haemophilus influenzae (NTHi) is a Gram negative microorganism residing in the human nasopharyngeal mucosa and occasionally causing infections of both middle ear and lower respiratory airways. A broadly protective vaccine against NTHi has been a long-unmet medical need,...

  • Multiplex PCR Assay for Detection of Pneumococcal Serotypes in Nasopharyngeal Samples of Healthy Children; Tehran, 2009-2010. Tabatabaei, S. Rafiei; Fallah, F.; Shiva, F.; Shamshiri, A. R.; Hajia, M.; Navidinia, M.; Karimi, A.; Rahbar, M. // Annual Review & Research in Biology;2014, Vol. 4 Issue 24, p3780 

    Background: Streptococcus pneumoniae (S. pneumoniae), is a major pathogen causing invasive disease, colonizes the nasopharynx constituting a potential source of infection in both children and adults. Aims: To identify the rate of pneumococcal nasopharyngeal colonization in healthy infants <2...

  • Certain Aspects of Silver and Silver Nanoparticles in Wound Care: A Minireview. Konop, Marek; Damps, Tatsiana; Misicka, Aleksandra; Rudnicka, Lidia // Journal of Nanomaterials;2/4/2016, p1 

    Resistance to antimicrobial agents by pathogenic bacteria has emerged in recent years and is a major health problem. In this context silver and silver nanoparticles (AgNP) have been known to have inhibitory and bactericidal effects and was used throughout history for treatment of skin ulcer,...

  • Invasive Haemophilus influenzae type b disease in elderly nursing hone residents two related cases. Heath, Timothy C.; Hewitt, Moira C. // Emerging Infectious Diseases;Apr-Jun97, Vol. 3 Issue 2, p179 

    Investigates two fatal cases of Haemophilus influenzae type b (Hib) in a western Sydney, Australian community nursing home. Reference to the two women sharing the same room; What isolates from blood cultures show; What the findings suggest.

  • Infections Due to Haemophilus influenzae Serotype E: Microbiological, Clinical, and Epidemiological Features. Campos, Jose; Roman, Federico; Perez-Vazquez, Maria; Oteo, Jesus; Aracil, Belen; Cercenado, Emilia // Clinical Infectious Diseases;9/15/2003, Vol. 37 Issue 6, p841 

    Discusses the microbiological, clinical and epidemiological features of infections due to Haemophilus influenzae serotype E. Identification of 26 H. influenzae serotype E (HiE) isolates with surveillance after introduction of H. influenzae serotype b vaccination in Spain; Resistance of Hie to...

  • Erratum.  // Clinical Infectious Diseases;May2011, Vol. 52 Issue 9, p1202 

    A correction to the article "Haemophilus influenzae type b vaccine failure in children is associated with inadequate production of high quality antibody" that was published in the January 15, 2008 issue of the periodical is presented.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics