TITLE

Hydrolysis of Ibuprofen Nitrile and Ibuprofen Amide and Deracemisation of Ibuprofen Using Nocardia corallina B-276

AUTHOR(S)
Ricardo Lievano; P�rez, Herminia In�s; Manjarrez, Norberto; Sol�s, Aida; Sol�s-Oba, Myrna
PUB. DATE
March 2012
SOURCE
Molecules;Mar2012, Vol. 17 Issue 3, p3148
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A novel application of whole cells of Nocardia corallina B-276 for the deracemisation of ibuprofen is reported. This microorganism successfully hydrolysed ibuprofen nitrile to ibuprofen amide, and ibuprofen amide to ibuprofen, using a suspension of cells in a potassium phosphate buffer solution (0.1 M, pH = 7.0). These results can be explained by the presence of NHase and amidase enzymes, but the reactions are not enantioselective and low ee values were obtained. However, (R)-ibuprofen was isolated with >99% ee by a deracemisation process catalysed by N. corallina B-276. This is the first report of this kind of catalysis with this microorganism.
ACCESSION #
74209026

Tags: NOCARDIA corallina;  IBUPROFEN;  HYDRATASES;  AMIDASES;  HYDROLYSIS;  MICROORGANISMS

 

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