TITLE

Design and In Vivo Immunogenicity of a Polyvalent Vaccine Based on SIVmac Regulatory Genes

AUTHOR(S)
Hel, Z.; Tryniszewska, E.; Tsai, W.P.; Johnson, J.M.; Harrod, R.; Fullen, J.; Kalyanaraman, V.S.; Altman, J.D.; McNally, J.; Karpova, T.; Felber, B.K.; Tartaglia, J.; Franchini, G.
PUB. DATE
September 2002
SOURCE
DNA & Cell Biology;Sep2002, Vol. 21 Issue 9, p619
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Most vaccine modalities for human immunodeficiency virus type 1 (HIV-1) tested for immunogenicity and efficacy in the SIVmac (simian immunodeficiency virus) macaque model do not include the viral regulatory proteins. Because viral regulatory proteins are expressed early during the virus life cycle and represent an additional source of antigens, their inclusion as a vaccine component may increase the overall virus-specific immune response in vaccinees. However, at least two of the early proteins, Tat and Nef, may be immunosuppressive, limiting their usefulness as components of an SIV vaccine. We have constructed a polyvalent chimeric protein in which the open reading frames for Tat and Nef have been reassorted and the nuclear localization sequence for Tat and Rev and the myristoylation site for Nef have been removed. The resulting DNA plasmid (pDNA-SIV-Retanef) (pDNA-SIV-RTN) encodes a protein of 55 kDa (Retanef) that localizes at the steady state in the cytoplasma of transfected cells. Both the DNA-SIV-RTN and the highly attenuated recombinant poxvirus vector NYVAC-SIV-RTN were demonstrated to be immunogenic in SIVmac251-infected macaques treated with ART as well as in naive macaques. An equivalent strategy may be used for the generation of polyvalent antigens encoding the regulatory proteins in a HIV-1 vaccine candidate.
ACCESSION #
7411862

 

Related Articles

  • HIV-1 Nef protein: An invitation to a kill. Cullen, Bryan R. // Nature Medicine;Sep99, Vol. 5 Issue 9, p985 

    New evidence demonstrates that HIV-1 Nef production by macrophages not only attracts T-lymphocytes but also renders them permissive for HIV-1 infection (pages 997?1003).

  • Optimization of the doxycycline-dependent simian immunodeficiency virus through in vitro evolution. Das, Atze T.; Klaver, Bep; Centlivre, Mireille; Harwig, Alex; Ooms, Marcel; Page, Mark; Almond, Neil; Fang Yuan; Piatak Jr., Mike; Lifson, Jeffrey D.; Berkhout, Ben // Retrovirology;2008, Vol. 5, Special section p1 

    Background: Vaccination of macaques with live attenuated simian immunodeficiency virus (SIV) provides significant protection against the wild-type virus. The use of a live attenuated human immunodeficiency virus (HIV) as AIDS vaccine in humans is however considered unsafe because of the risk...

  • Simian Virus Large T Antigen Interacts with the N-Terminal Domain of the 70 kD Subunit of Replication Protein A in the Same Mode as Multiple DNA Damage Response Factors. Ning, Boting; Feldkamp, Michael D.; Cortez, David; Chazin, Walter J.; Friedman, Katherine L.; Fanning, Ellen // PLoS ONE;Feb2015, Vol. 10 Issue 2, p1 

    Simian virus 40 (SV40) serves as an important model organism for studying eukaryotic DNA replication. Its helicase, Large T-antigen (Tag), is a multi-functional protein that interacts with multiple host proteins, including the ubiquitous ssDNA binding protein Replication Protein A (RPA). Tag...

  • Pirate Primates in Uncharted Waters: Lymphocyte Transfers in Unrelated, MHC-Matched Macaques. Burwitz, Benjamin J.; Greene, Justin M.; O'Connor, David H. // Current HIV Research;Jan2009, Vol. 7 Issue 1, p51 

    An HIV vaccine remains elusive despite the concerted efforts of investigators and clinicians over the past two decades. Animal models are regularly used to obtain new insights on disease pathogenesis and have become invaluable tools in the translation of treatments from basic research...

  • Infection of mesangial cells with HIV and SIV: Identification of GPR1 as a coreceptor. Tokizawa, Shigemi; Shimizu, Nobuaki; Hui-Yu, Liu; Deyu, Fang; Haraguchi, Yuji; Oite, Takashi; Hoshino, Hiroo // Kidney International;Aug2000, Vol. 58 Issue 2, p607 

    Infection of mesangial cells with HIV and SIV: Identification of GPR1 as a coreceptor. Background. Mesangial cells are an important component of the glomerulus. Dysfunction of mesangial cells is thought to be involved in the development of human immunodeficiency virus type 1 (HIV-1)-associated...

  • Will wild chimp pee tell all about HIV? Brown, Phyllida // New Scientist;09/11/99, Vol. 163 Issue 2203, p25 

    Focuses on a report in the September 4, 1999 issue of the `New Scientist,' on research into the origins of the HIV through the construction of a family tree fir chimpanzee forms of the simian immunodeficiency virus (SIV). Rationale behind the study of SIV; Relationship between SIV and HIV.

  • Good news on AIDS. Costello, Emily // Science World;2/9/96, Vol. 52 Issue 9, p6 

    Reports on two discoveries in the battle against AIDS. Persistence of good health of people in Australia for 14 years after being infected with HIV; Possible use of genetically defective strain of HIV as vaccine; Effectiveness of a drug in stopping simian immuno-deficiency virus (SIV);...

  • Animal Models Used for the Evaluation of Antiretroviral Therapies. P. Dias, Andreia S.; Bester, Megan J.; Britz, Rozane F.; Apostolides, Zeno // Current HIV Research;Oct2006, Vol. 4 Issue 4, p431 

    Several animal models for the study of HIV/AIDS have been established and characterized and have been widely used to study the pathogenesis of HIV/AIDS as well as vaccine development. The purpose of this study was to review the literature and identify the animal models most frequently used for...

  • Researchers track ancestry of HIV to monkey meat.  // Gay & Lesbian Times;6/19/2003, Issue 808, p28 

    Reports that the ancestry of the HIV virus has been traced to two strains of virus found in monkeys in Africa. Ways by which the viruses have been passed into chimpanzees; Genetic pattern of the simian immunodeficiency virus.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics