TITLE

RP-HPLC Method Development and Validation for Simultaneous Estimation of Amlodipine besylate,Valsartan and Hydrochlorothiazide in Tablet Dosage Form

AUTHOR(S)
Younus, Mohammad; Reddy, T. Karnaker; Reddy, Y. Ravindra; Arif, Md. Fasiuddin
PUB. DATE
November 2010
SOURCE
Journal of Pharmacy Research;Nov2010, Vol. 3 Issue 11, p2647
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A simple, specific, accurate and precise Reverse Phase High Performance Liquid Chromatographic method was developed for simultaneous estimation of Amlodipine Besylate (AB), Valsartan (VAT) and Hydrochlorothiazide (HTZ) in tablet dosage form on RP C-18 Column (Hypersil 250*4.6 mm) using Acetonitrile: Mixed Phosphate buffer (6.8 pH) (55:45) (buffer was prepared by mixing the equal proportions of 0.01M Potassium dihydrogenphosphate and 0.001M Dipotassium hydrogenphosphate. pH was adjusted with Orthophosphoric acid) as mobile phase. The flow rate was 1.0 ml/min and effluent was monitored at 237nm. The retention time for VAT, HTZ and AB was found to be as 2.28, 2.99 and 4.57 respectively. Proposed method was validated for Precision, Accuracy, Linearity range, Robustness and Ruggedness.
ACCESSION #
74101597

 

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