Surface-Enhanced Raman Scattering Spectroscopy of Topotecan–DNA Complexes: Binding to DNA Induces Topotecan Dimerization

Mochalov, K. E.; Strel’tsov, S. A.; Ermishov, M. A.; Grokhovskiı, S. L.; Zhuze, A. L.; Ustinova, O. A.; Sukhanova, A. V.; Nabiev, I. R.; Oleınikov, V. A.
September 2002
Optics & Spectroscopy;Sep2002, Vol. 93 Issue 3, p416
Academic Journal
The interaction of topotecan (TPT), antitumor inhibitor of human DNA topoisomerase I, with calf thymus DNA was studied by surface-enhanced Raman scattering (SERS) spectroscopy. The SERS spectra of TPT are found to depend on its concentration in solution, which is associated with the dimerization of TPT. The spectral signatures of dimerization are identified. It is shown that binding to DNA induces the formation of TPT dimers. The formation of DNA-TPT-TPT-DNA complexes is considered as one of the possible mechanisms of human DNA topoisomerase I inhibition.


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