TITLE

Introduction of A Novel Binary Solvent System For The Determination of Pesticides By High-Performance Liquid Chromatography With Ultra Violet Detection

AUTHOR(S)
Mishra, R. Soni; Verma, Pratibha; Namboodiry, Varsha; Bhagwat, Ashok; Bhoir, Suvarna I.
PUB. DATE
October 2011
SOURCE
International Journal of Research in Pharmacy & Science;Oct2011, Vol. 1 Issue 3, p148
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The present research intends to confirm successful implication of propylene carbonate and methanol in the ratio of 60:40 (Solvent-X) as a mobile phase component in the analysis of carbendazim, isoproturon and pendimethalin by high pressure liquid chromatography (HPLC) with Ultra Violet (UV) detection. Chromatography was performed on (250 x 4.6 mm), 5-µm, Inertsil ODS C18 column with a flow rate of 1.0 ml/min for all the three pesticides with varying compositions of mobile phase. UV detection was carried out at 254 nm, 230 nm and 240 nm for Carbendazim, Isoproturon and Pendimethalin respectively. For each pesticide the method was validated two times initially with acetonitrile in the mobile phase and later by substituting it with Solvent-X maintaining all other chromatographic conditions unvaried to correlate the discrepancy in the figures of merit and other validation parameters. However it was ascertained that both the methods provide comparable results. Linearity was established between a concentration range of 25.0 to 75.0 µg/ml for Carbendazim, for Isoproturon it was 0.75 to 11.25 µg/ml and for Pendimethalin 0.30 to 4.50 µg/ml using internal standard method with a correlation coefficient of 0.9995, 0.9994 and 0.9994 with acetonitrile and 0.9998, 0.9984 and 0.9995 with Solvent-X.
ACCESSION #
73921205

 

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