TITLE

RAPID PLANT REGENERATION FROM NODAL EXPLANTS OF Spilanthes acmella (L.) MURR. - AN ENDANGERED MEDICINAL PLANT

AUTHOR(S)

Yadav, Kuldeep; Singh, Narender

PUB. DATE

March 2012

SOURCE

Analele Universitatii din Oradea, Fascicula Biologie;2012, Vol. 19 Issue 1, p35

SOURCE TYPE

Academic Journal

DOC. TYPE

Article

ABSTRACT

Excised nodal explants of Spilanthes acmella (L.) Murr., 'Toothache Plant' proliferate rapidly in vitro on MS medium containing 0.5- 2.0 mg/l of BAP. Rapid and prolific shoot proliferation occurred. Regenerated shoots vary considerably in size (3-10 cm long) and relative stage of development, with some (50%) producing adventitious roots without transferal to a separate rooting medium. With maximum possibility of adventitious roots induction was induced from middle order nodes (3rd to 5th node from apex) obtained from 3 months old in vivo plant on full-strength MS medium supplemented with 1.0 mg/l BAP under the photoperiod of 18-h. The possibility of adventitious roots induction directly from regenerated shoot was greatly influenced by the concentration of BAP, photoperiod, age of donor plant and nodal position on stem.

ACCESSION #

73898776

Tags: SPILANTHES;  MEDICINAL plants;  ENDANGERED plants;  REGENERATION (Botany);  PLANT photoperiodism

 

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