Evaluation of Multiplicative Killing Effect of C.odorata extracts and β-lactam antibiotics against β-lactamase Producing bacteria, isolated from Selected Hospitals in Ilorin Metropolis

Ajao, A. T.; Ajadi, T. S.; Oyelowo, M. S.
August 2011
Annals of Biological Research;2011, Vol. 2 Issue 4, p76
Academic Journal
The occurrence of β-lactamase producing bacteria in selected private hospitals, using iodometric method was evaluated The multiplicative killing effect of combinations of the crude methanolic extract of the leaves of Chromolaena odorata and β-lactam antibiotics were studied against β-lactamase producing bacteria. The interaction between methanol extracts of C odorata, alone and then synergy testing of these extracts with known antimicrobial agents of cell wall synthesis inhibition mechanism using well-diffusion and broth medium method. The results of the research using well-diffusion method demonstrated that these C.odorata showed in vitro interactions between penicillin G and the extracts were synergistic against E.coli , while antagonism was observed in cephalosporin against Pseudomonas aeruginosa and Proteus vulgaris. Broth medium method also showed synergistic effects in the combination of antibiotics and C.odorata extracts with significant correlation in the MICs and MBCs values of the test antibiotics with zone of inhibition against the test strains except those that showed antagonism effect. The effect of combinations of the extract and some antibiotics on their resistance Enzyme cleavage of β-lactam rings mechanism was evaluated in an effort to explore new bioactive molecules of high therapeutic values


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