Partial sequencing and phylogenetic analysis of Soybean mosaic virus isolated in Ukraine

Sherepitko, D. V.; Budzanivska, I. G.; Polischuk, V. P.; Boyko, A. L.
December 2011
Biopolymers & Cell;2011, Vol. 27 Issue 6, p472
Academic Journal
The aim of the present study is to compare the biological and molecular properties of Ukrainian soybean mosaic virus (SMV) isolates with those of known strains or isolates from other countries, and to trace their possible origin. The methods of mechanical inoculation, reverse transcription polymerase chain reaction, DNA sequencing and phylogenetic analysis have been used. Results. Five SMV isolates have been collected and biologically purified from breeding plots in Vinnitsa region of Ukraine. It has been found that all these isolates show the same reaction patterns when infecting 11 differential soybean cultivars. Phylogenetic analysis of sequences of the coat protein coding region and P1 coding region revealed strong genetic relationships between representative Ukrainian (UA1Gr) and SMV-VA2 isolates which together were sorted in one clade with G2 strain. The investigation of sequence identity showed that different genomic regions of SMV were under different evolutionary constraints. Conclusions. SMV, isolated in Ukraine for the first time, belongs to the G2 strain group that is widespread in North America. The SMV isolates obtained in this work may be employed in the Ukrainian national breeding programs to create soybean with durable virus resistance.


Related Articles

  • Callose deposition at plasmodesmata is a critical factor in restricting the cell-to-cell movement of Soybean mosaic virus. Li, Wenlong; Zhao, Yongshan; Liu, Chunji; Yao, Guibin; Wu, Sisi; Hou, Chunyan; Zhang, Mengchen; Wang, Dongmei // Plant Cell Reports;May2012, Vol. 31 Issue 5, p905 

    Callose is a β-l,3-glucan with diverse roles in the viral pathogenesis of plants. It is widely believed that the deposition of callose and hypersensitive reaction (HR) are critical defence responses of host plants against viral infection. However, the sequence of these two events and their...

  • Intra-host competition and interactions between Soybean mosaic virus (SMV) strains in mixed-infected soybean. Tae-Young Hwang; Soon-Chun Jeong; Oksun Kim; Hyang-Mi Park; Seuk-Ki Lee; Min-Jung Seo; Man-Soo Choi; Yu-Young Lee; Hong-Tai Yun; Young-Up Kwon; Wook Han Kim; Yul-Ho Kim // Australian Journal of Crop Science;2011, Vol. 5 Issue 11, p1379 

    Over the past two decades, the dominant Soybean mosaic virus (SMV) strain in South Korea has changed from G5 to G7H. To examine the dominance of G7H, intra-host competition between G7H and G5 was evaluated in soybean plants infected with a mixture of SMV strains. The distribution patterns of the...

  • Inheritance and Allelism of Resistance to Soybean Mosaic Virus in Zao18 Soybean From China. Liao, l.; Chen, P.; Buss, G. R.; Yang, Q.; Tolin, S. A. // Journal of Heredity;Nov/Dec2002, Vol. 93 Issue 6, p447 

    Examines the reactions of Chinese soybean cultivar Zao18 to soybean mosaic virus (SMV) strains. Background on SMV; Analysis of the inheritance of soybean resistance to SMV; List of gene loci for SMV resistance.

  • Analysis of the entire genomes of thirteen TT virus variants classifiable into the fourth and fifth genetic groups, isolated from viremic infants. Peng, Y. H.; Nishizawa, T.; Takahashi, M.; Ishikawa, T.; Yoshikawa, A.; Okamoto, H. // Archives of Virology;Jan2002, Vol. 147 Issue 1, p21 

    Summary. TT virus (TTV) DNA in serum samples obtained from 24 TTV-infected infants was amplified by polymerase chain reaction (PCR) with inverse primers derived from the untranslated region. The amplified PCR products were molecularly cloned; six clones each were analyzed. Seventy-six (53%) of...

  • Subtyping of Y-chromosomal haplogroup E-M78 (E1b1b1a) by SNP assay and its forensic application. Caratti, S.; Gino, S.; Torre, C.; Robino, C. // International Journal of Legal Medicine;Jul2009, Vol. 123 Issue 4, p357 

    The continual discovery of new single-nucleotide polymorphisms (SNPs) has led to an increased resolution of the Y chromosome phylogeny. Some of these Y-SNPs have shown to be restricted to small geographical regions and therefore may prove useful in the forensic field as tools for the prediction...

  • Hepatitis B virus genotypes circulating in Brazil: molecular characterization of genotype F isolates. Mello, Francisco C. A.; Souto, Francisco J. D.; Nabuco, Leticia C.; Villela-Nogueira, Cristiane A.; Coelho, Henrique Sergio M.; Franz, Helena Cristina F.; Saraiva, Joao Carlos P.; Virgolino, Helaine A.; Motta-Castro, Ana Rita C.; Melo, Mabel M. M.; Martins, Regina M. B.; Gomes, Selma A. // BMC Microbiology;2007, Vol. 7, p103 

    Background: Hepatitis B virus (HBV) isolates have been classified in eight genotypes, A to H, which exhibit distinct geographical distributions. Genotypes A, D and F are predominant in Brazil, a country formed by a miscegenated population, where the proportion of individuals from Caucasian,...

  • First molecular characterization of feline immunodeficiency virus in Turkey. iğdem^O&;#x011F;uzoğlu, Tuba Ç; Timurkan, Mehmet Özkan; Muz, Dilek; Kudu, Ayşegül; Numanbayraktaroğlu, Başak; Sadak, Seda; Burgu, Ibrahim // Archives of Virology;Nov2010, Vol. 155 Issue 11, p1877 

    In this study, strains of feline immunodeficiency virus (FIV), designated TR-D, TR-Mo and TR-Mi, isolated from three cats in Turkey, were characterized. PCR products (859 bp) from the envelope (env) gene region were amplified and sequenced, and possible geographical differences in the env gene...

  • Phylogenetic diversity of dissimilatory ferric iron reducers in paddy soil of Hunan, South China. Xin-Jun Wang; Jing Yang; Xue-Ping Chen; Guo-Xin Sun; Yong-Guan Zhu // Journal of Soils & Sediments: Protection, Risk Assessment, & Rem;Dec2009, Vol. 9 Issue 6, p568 

    Purpose Dissimilatory iron-reducing bacteria have been described by both culture-dependent and -independent methods in various environments, including freshwater, marine sediments, natural wetlands, and contaminated aquifers. However, little is known about iron-reducing microbial communities in...

  • Detection and molecular characterization of phytoplasma associated with chickpea phyllody disease in south India. Pallavi, M.; Ramappa, H.; Shankarappa, K.; Rangaswamy, K.; Wickramaarachchi, W.; Maruthi, M. // Phytoparasitica;Jul2012, Vol. 40 Issue 3, p279 

    Chickpea ( Cicer arietinum L.) plants showing typical symptoms of infection by a phytoplasma that causes phyllody disease have been commonly observed in recent years in parts of south India. The symptoms included pale green leaves, bushy appearance due to excessive stunting of shoots, reduced...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics