A Sensitive RP-HPLC Method for Simultaneous Estimation of Diethylcarbamazine and Levocetirizine in Tablet Formulation

Reddy, J. Mahesh; Jeyaprakash, M. R.; Madhuri, K.; Meyyanathan, S. N.; Elango, K.
May 2011
Indian Journal of Pharmaceutical Sciences;May/Jun2011, Vol. 73 Issue 3, p320
Academic Journal
A simple, sensitive and reproducible method was developed and validated for the simultaneous estimation of diethylcarbamazine and levocetirizine in its tablet formulation by reverse phase high performance liquid chromatography using Waters1515 HPLC with UV detector at the λmax of 224 nm, using Princeton Sphere-100 C18 (250x4.6 mm. 5 µ) column. The mobile phase used was 20mM potassium dihydrogen orthophosphate buffer (pH: 3.2):acetonitrile (50:50 v/v) with isocratic flow (flow rate 1 ml/min) and the pH was adjusted with orthophosphoric acid. Losartan potassium was used as an internal standard. The compounds diethylcarbamazine, levocetirizine and losartan potassium were eluted at 2.12, 4.27 and 5.96 min, respectively. The peaks were eluted with better resolution. The method was accurate with assay values of 96.32 and 93.04% w/w, precise (%RSD) with intra-day 1.72 and 1.89 and inter-day 1.85 and 1.92, recoveries 102.86 and 101.1% w/w, which are very sensitive with limit of detections (LOD)'s 75, 50 ng/ml and limit of quantification (LOQ)'s 100, 75 ng/ml and linear with R² values 0.994 in the range of 5 to 30 µg/ml 0.1 to 1 µg/ml for diethylcarbamazine and levocetirizine, respectively. Hence this method can be applied for quantification of different formulations containing diethylcarbamazine and levocetirizine simultaneously.


Related Articles

  • Analytical Method for the Simultaneous Estimation of Hydrochlorothiazide and Metoprolol Tartrate using RP HPLC. Rawool, N. D.; Venkatchalam, A. // Indian Journal of Pharmaceutical Sciences;Mar/Apr2011, Vol. 73 Issue 2, p219 

    The present study deals with the estimation by RP-HPLC of two different drug components hydrochlorothiazide and metoprolol tartrate present in a tablet formulation. It is a simple, fast, precise and accurate high performance liquid chromatographic method. It is performed using phosphate buffer...

  • Spectrophotometric Method for Estimation of Metformin Hydrochloride. Mubeen, G.; Noor, Khalikha; Vimala, M. N. // International Journal of ChemTech Research;Apr2010, Vol. 2 Issue 2, p1186 

    A simple Spectrophotometric method has been developed and validated for the estimation of Metformin hydrochloride in bulk and in tablet formulation. The primary amino group of Metformin hydrochloride was oxidized using hydrogen peroxide to form a yellow chromogen , which is determined...


    A Simple precise and accurate method was developed and validated for the simultaneous analysis of chlorthalidone and irbesarton in tablet formulations. The method has been shown adequate separation of the two ingredients from each other. The chromatographic separation was achieved on a reverse...

  • RP-HPLC method development and validation for determination of dissolution and assay of sildenafil citrate tablets. Mallu, Useni Reddy; Reddy, K. Hussain; Bobbarala, Varaprasad; Penumajji, Somasekhar // Journal of Pharmacy Research;Mar2010, Vol. 3 Issue 3, p631 

    The aim of this study is to development and validation of a stability indicating RP-HPLC method for the determination of dissolution and assay of Sildenafil citrate tablets 50mg and 100mg using RP-HPLC with UV detector. Sildenafil citrate and related substances were baseline separated and...

  • A novel RP-HPLC method for the quantification of lornoxicam in formulations. Devanaboyina, Narendra; Satyanarayana, T.; Rao, B. Ganga // Journal of Pharmacy Research;Dec2011, Vol. 4 Issue 12, p4741 

    A simple, precise and accurate RP-HPLC method was developed and validated for rapid assay of Lornoxicam in tablet dosage form. Isocratic elution at a flow rate of 1.0ml/min was employed on a symmetry Chromosil C18 (250x4.6mm, 5µm in particle size) at ambient temperature. The mobile phase...

  • NEW VALIDATED RP-HPLC METHOD FOR THE ESTIMATION OF CARVEDILOL IN PHARMACEUTICAL FORMULATION. .M.V, Basaveswara Rao; A. V. D, Nagendrakumar; .M, Yedukondalu; .B.V, RAMAN // International Journal of Pharmacy & Pharmaceutical Sciences;Mar2012, Vol. 4 Issue 2, p353 

    Asimple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Carvedilol in Bulk and Pharmaceutical tablet Formulation. Isocratic elution at a flow rate of 1.0ml/min was employed on symmetry C18 (250 mm x 4.6 mm, 5μm) Column at ambient...

  • High Performance Liquid Chromatographic Method for Estimation of Repaglinide in Tablet Dosage Forms. Jiladia, Meeta A.; Pandya, S. S.; Jiladia, Ashok G. // International Journal of Pharma & Bio Sciences;Apr-Jun2010, Vol. 1 Issue 2, p1 

    A simple and sensitive, HPLC method has been developed and validated for the quantitative estimation of repaglinide in its single component tablet formulations (2 mg). Determination was performed using a Thermo BDS C18 column with mobile phase phosphate buffer: acetonitrile (pH 6.0; 45:55, v/v),...

  • HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF LAMIVUDINE AND STAVUDINE IN BULK AND COMBINED TABLET DOSAGE FORM. Kumaraswamy, G.; Rajendra Kumar, J. M.; Sheshagiri Rao, J. V. L. N.; Vinay Kumar, D.; Prabakar, D.; Ashok Kumar, U. // International Journal of Pharmacy & Pharmaceutical Sciences;Jul2011, Vol. 3 Issue 3, p142 

    A Simple, accurate and precise HPLC method was developed for the simultaneous estimation of Lamivudine and Stavudine in bulk and pharmaceutical dosage forms. The quantification was carried out using symmetry C-18, Thermo (100x4.6,), i.d, and 3.5 μm particle size in isocratic mode, with mobile...

  • A Simple RP-HPLC Method for Quantitation of Itopride HCl in Tablet Dosage Form. Rajan, V. S. Thiruvengada; Saleem, T. S. Mohamed; Ramkanth, S.; Alagusundaram, M.; Ganaprakash, K.; Madhusudhana Chetty, C. // Journal of Young Pharmacists;2010, Vol. 2 Issue 4, p410 

    An isocratic reversed phase high-performance liquid chromatographic method with ultraviolet detection at 220 nm has been developed for the quantification of itopride hydrochloride in tablet dosage form. The quantification was carried out using C8 column (250 mm × 4.6 mm), 5-μm particle...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics