TITLE

The ribosomal protein L19 mRNA is induced by copper exposure in the swordtail fish, Xiphophorus helleri

AUTHOR(S)

Aliza, Dwinna; Tey, Chiew-Lian; Ismail, Ida; Kuah, Meng-Kiat; Shu-Chien, Alexander; Muhammad, Tengku

PUB. DATE

April 2012

SOURCE

Molecular Biology Reports;Apr2012, Vol. 39 Issue 4, p4823

SOURCE TYPE

Academic Journal

DOC. TYPE

Article

ABSTRACT

Teleosts are useful vertebrate model species for understanding copper toxicity due to the dual entry route for copper intake via the gills and intestine. In this present study, we utilized the differential display reverse transcription-polymerase chain reaction to isolate potential novel hepatic genes induced by sublethal copper exposure in the freshwater swordtail fish, Xiphophorus helleri. Full length cloning of a cDNA fragment induced by copper exposure to 1 μg/ml during 24 h resulted in the positive identification of a hepatic ribosomal protein L19 (RPL19) gene. Further characterization of this gene revealed that its transcriptional expression was dependent on dosage and time of copper exposure. This study describes for the first time the involvement of RPL19 in copper toxicity, probably as a result of increase in ribosome synthesis rate to support activities such as cellular protein translation, transcriptional activation and mRNA stabilization during sublethal copper exposure.

ACCESSION #

72412928

Tags: OSTEICHTHYES -- Research;  COPPER -- Toxicology;  REVERSE transcriptase polymerase chain reaction;  MESSENGER RNA;  ANIMAL models in research;  VERTEBRATES;  RIBOSOMAL proteins

 

Related Articles

• Coexpression of an unusual form of the EWS�WT1 fusion transcript and interleukin 2/15 receptor {szligbeta}mRNA in a desmoplastic small round cell tumour. Y Nakanishi // Journal of Clinical Pathology;Oct2006, Vol. 59 Issue 10, p1108 

  BACKGROUND: The {szligbeta} chain of the interleukin 2/15 receptor (IL-2/15R{szligbeta}) is induced by the expression of the EWS�WT1. A case of desmoplastic small round cell tumour (DSRCT) expressing only an unusual EWS-WT1 treated by us is reported here. Aim: To characterise an unusual form...

• Transcriptome-wide identification and characterization of miRNAs from Pinus densata.  // BMC Genomics;2012, Vol. 13 Issue 1, p132 

  The article presents a study which aims at an identification and characterization in the transcriptome of microRNA (miRNA) from Pinus (P.) densata. It describes the methodology of the study in which Illumina sequencing technology was used to obtain gene sequences from a P. densata mRNA library...

• The possible role of ribosomal protein S6 kinase 4 in the senescence of endothelial progenitor cells in diabetes mellitus.  // Cardiovascular Diabetology;2012, Vol. 11 Issue 1, p12 

  The article examines the role of ribosomal protein S6 kinase 4 in the senescence of endothelial progenitor cells (EPCs) in patients with diabetes mellitus (DM). It mentions the dysfunction of EPCs has been assumed as an important cause of DM. Further it analyses the evidence-based hypothesis...

• The HIV-1 Nef Protein Interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA.  // Virology Journal;2012, Vol. 9 Issue 1, p103 

  The article discusses the study which investigates the interaction of the HIV-1 Nef-encoded protein with the components of the 40S small ribosomal unit and the 18S rRNA. The study used an in vitro translation assay to determine the effect of Nef on translation. The study performed...

• Neonatal manifestation of multiple sulfatase deficiency. Busche, Andreas; Hennermann, Julia B.; B�rger, Friederike; Proquitt�, Hans; Dierks, Thomas; von Arnim-Baas, Annabel; Horn, Denise // European Journal of Pediatrics;Aug2009, Vol. 168 Issue 8, p969 

  Multiple sulfatase deficiency is biochemically characterized by the accumulation of sulfated lipids and acid mucopolysaccharides. We report clinical, biochemical, and molecular findings in a female newborn affected with a severe form of multiple sulfatase deficiency (Mendelian Inheritance in Man...

• Titer improvement of iso-migrastatin in selected heterologous Streptomyces hosts and related analysis of mRNA expression by quantitative RT-PCR. Dong Yang; Xiangcheng Zhu; Xueyun Wu; Zhiyang Feng; Lei Huang; Ben Shen; Zhinan Xu // Applied Microbiology & Biotechnology;Mar2011, Vol. 89 Issue 6, p1709 

  iso-Migrastatin (iso-MGS) has been actively pursued recently as an outstanding candidate of antimetastasis agents. Having characterized the iso-MGS biosynthetic gene cluster from its native producer Streptomyces platensis NRRL 18993, we have recently succeeded in producing iso-MGS in five...

• Influence of Aging on the Dopaminergic Neurons in the Substantia Nigra Pars Compacta of Rats. Jin Gao; Hui Miao; Cheng-hua Xiao; Yu Sun; Xia Du; Hong-hua Yuan; Hong-li Yu; Dianshuai Gao // Current Aging Science;Feb2011, Vol. 4 Issue 1, p19 

  To study the relationship between aging and the vulnerability of substantia nigra pars compacta (SNc) tyrosine hydroxylase immunoreactive positive (TH+) dopaminergic (DA) neurons. We determined the number of TH+ DA neurons in aged rats (24 mon) compared to adult rats (5 mon) using...

• Effects of lipopolysaccharides extracted from Porphyromonas endodontalis on the expression of IL-1� mRNA and IL-6 mRNA in osteoblasts. YANG Di; LI Ren; QIU Li-hong; LI Chen // Shanghai Journal of Stomatology;Apr2009, Vol. 18 Issue 2, p194 

  PURPOSE: To quantify the IL-1� mRNA and IL-6 mRNA expression induced by lipopolysaccharides (LPS) extracted from Porphyromonas endodontalis (P.e) in osteoblasts, and to relate P.e-LPS to bone absorption pathogenesis in lesions of chronical apical periodontitis. METHODS: MG63 was treated with...

• mRNA profiling of the cancer degradome in oesophago-gastric adenocarcinoma. Baren, J P; Stewart, G D; Stokes, A; Gray, K; Pennington, C J; O'Neill, R; Deans, D A C; Paterson-Brown, S; Riddick, A C P; Edwards, D R; Fearon, K C H; Ross, J A; Skipworth, R J E // British Journal of Cancer;6/26/2012, Vol. 107 Issue 1, p143 

  Background:Degradation of the extracellular matrix is fundamental to tumour development, invasion and metastasis. Several protease families have been implicated in the development of a broad range of tumour types, including oesophago-gastric (OG) adenocarcinoma. The aim of this study was to...

Read the Article