TITLE

Construction of the industrial ethanol-producing strain of Saccharomyces cerevisiae able to ferment cellobiose and melibiose

AUTHOR(S)
Zhang, L.; Guo, Z.; Ding, Z.; Wang, Z.; Shi, G.
PUB. DATE
March 2012
SOURCE
Applied Biochemistry & Microbiology;Mar2012, Vol. 48 Issue 2, p216
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The gene mel1, encoding α-galactosidase in Schizosaccharomyces pombe, and the gene bgl2, encoding and α-glucosidase in Trichoderma reesei, were isolated and co-expressed in the industrial ethanolproducing strain of Saccharomyces cerevisiae. The resulting strains were able to grow on cellobiose and melibiose through simultaneous production of sufficient extracellular α-galactosidase and β-glucosidase activity. Under aerobic conditions, the growth rate of the recombinant strain GC1 co-expressing 2 genes could achieve 0.29 OD600 h and a biomass yield up to 7.8 g l dry cell weight on medium containing 10.0 g l cellobiose and 10.0 g l melibiose as sole carbohydrate source. Meanwhile, the new strain of S. cerevisiae CG1 demonstrated the ability to directly produce ethanol from microcrystalline cellulose during simultaneous saccharification and fermentation process. Approximately 36.5 g l ethanol was produced from 100 g of cellulose supplied with 5 g l melibose within 60 h. The yield (g of ethanol produced/g of carbohydrate consumed) was 0.44 g/g, which corresponds to 88.0% of the theoretical yield.
ACCESSION #
72091661

 

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