TITLE

Development of a new immunoassay for the accurate determination of anti-infliximab antibodies in inflammatory bowel disease

AUTHOR(S)
Imaeda, Hirotsugu; Andoh, Akira; Fujiyama, Yoshihide
PUB. DATE
February 2012
SOURCE
Journal of Gastroenterology;Feb2012, Vol. 47 Issue 2, p136
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: The formation of antibodies to infliximab (ATIs) is closely associated with the loss of response to infliximab in patients with inflammatory bowel disease (IBD). We evaluated the clinical utility of a novel method to measure serum ATI levels in the presence of infliximab. Methods: ATI levels were measured using a novel immunoassay and the conventional method in 58 patients with Crohn's disease (CD) under infliximab maintenance therapy. The serum infliximab trough levels were determined by enzyme-linked immunosorbent assay. Results: ATIs were detected in 16 out of 58 patients (27.6%) by the new method, but the conventional method detected only 2 patients (3.4%) who had the two highest ATI titers assayed by the new method. The presence of ATIs in the samples positive by the new method but negative by the conventional method was confirmed by Western blot analysis. Western blotting analysis also indicated that the new method could restore the binding capacities of the ATIs whose recognition sites were occupied by free infliximab. In the new method, the addition of infliximab to the samples dose-dependently blocked the detection of ATIs. Patients positive for ATIs had significantly lower serum trough levels of infliximab ( P < 0.01) and significantly higher clinical activity scores ( P < 0.001) as compared with patients negative for ATI. Conclusions: The new method makes it possible to measure serum ATI levels in the presence of infliximab. This method is useful for deciding the optimal management strategies for IBD patients with loss of response to infliximab.
ACCESSION #
71815393

 

Related Articles

  • Chapter 12: Serology. Van Regenmortel, Marc H. V.; Khan, Jawaid A.; Dijkstra, Jeanne // Handbook of Plant Virology;2006, p145 

    The article focuses on immunoassays or immunochemical techniques. An enzyme-linked immunosorbent assay is a solid-phase immunoassay on a plastic surface and the reaction is detected by means of enzyme-labeled antibodies. In plant virology, various immunoblotting assays are commonly used, such as...

  • Evidence for HTLV-III infection in prostitutes in Tamil Nadu (India). Simoes, Eric A. F.; Babu, P. George; John, T. Jacob; Nirmala, S.; Solomon, Suniti; Lakshminarayana, C. S.; Quinn, Thomas C. // Indian Journal of Medical Research;Oct2012, Vol. 136 Issue 4, p335 

    Antibody to human T-lymphotropic virus type III has been detected by the enzyme-linked immunosorbent assay and confirmed by western blot analysis in 10 among 102 prostitutes in Tamil Nadu (India).

  • A multicentre evaluation of the new Beckman Coulter anti-Müllerian hormone immunoassay (AMH Gen II). Wallace, A. M.; Faye, S. A.; Fleming, R.; Nelson, S. M. // Annals of Clinical Biochemistry;Jul2011, Vol. 48 Issue 4, p370 

    Background: The measurement of anti-Müllerian hormone (AMH) has been by two commercial enzyme-linked immunosorbent (ELISA) assays: Diagnostics Systems Laboratory (DSL 10-14400) and Immunotech (A11893 IVD EU only). Beckman Coulter has developed a new assay for AMH (AMH Gen II A79765), which...

  • Total serum IgE quantification by microfluidic ELISA using magnetic beads. Proczek, Gaëlle; Gassner, Anne-Laure; Busnel, Jean-Marc; Girault, Hubert // Analytical & Bioanalytical Chemistry;May2012, Vol. 402 Issue 8, p2645 

    The present work reports on the quantification of total IgE in human serum using a microanalytical device whose fluidics is driven by gravity and capillary forces only. Thanks to the eight parallel microchannels in each microchip, calibration and sample analysis are performed simultaneously. A...

  • A NOVEL FULLY AUTOMATED ENZYME IMMUNO-ASSAY FOR THE DETECTION OF ANTIBODIES AGAINST HUMAN ß-2-GLYCOPROTEIN I.  // Lupus;Jan2001 Supplement 1, Vol. 10, pS60 

    An abstract of the study entitled "A Novel Fully Automated Enzyme Immunoassay for the Detection of Antibodies Against Human ß-2-Glycoprotein I" is presented. The study examines the blood serum of 850 patients with or without autoimmune diseases to detect anti-ß2-glycoprotein I through...

  • Discrepancies between a new highly sensitive Toxoplasma gondii ELISA assay and other reagents: interest of Toxo IgG Western blot. Lesl�, F.; Touafek, F.; Fekkar, A.; Mazier, D.; Paris, L. // European Journal of Clinical Microbiology & Infectious Diseases;Oct2011, Vol. 30 Issue 10, p1207 

    Immunodiagnostic assays are commonly used to screen for maternal toxoplasmic seroconversion during pregnancy. The introduction to the market of a new highly sensitive IgG assay, the Elecsys Toxo IgG test, has resulted in discrepancy issues with other immunoassays because of a lack of...

  • Multiple immunoassay systems are negatively interfered by circulating cardiac troponin I autoantibodies. Tang, Gusheng; Wu, Yu; Zhao, Weiguo; Shen, Qian // Clinical & Experimental Medicine;Mar2012, Vol. 12 Issue 1, p47 

    Circulating cardiac troponin I (cTnI) autoantibodies have recently been detected in more and more patients with myocardial injury. In the present study, a total of 121 patients with acute myocardial infarction (AMI) were screened for cTnI autoantibodies using an indirect ELISA. Positive results...

  • A novel monoclonal antibody immunoassay for the detection of human serum hepcidin. Schwarz, Peggy; Strnad, Pavel; Figura, Guido; Janetzko, Alfred; Krayenbühl, Pierre; Adler, Guido; Kulaksiz, Hasan // Journal of Gastroenterology;May2011, Vol. 46 Issue 5, p648 

    Background: Hepcidin is a liver-derived peptide hormone regulating iron metabolism. Changes in the expression of hepcidin are known to be the key pathogenic factors in hereditary hemochromatosis and are associated with infection and inflammation. To better understand the hormone's function in...

  • Comparison of a rapid immunoassay for antibodies to the C6 antigen with conventional tests for antibodies to Borrelia burgdorferi in dogs in Europe. Gerber, B.; Haug, K.; Eichenberger, S.; Reusch, C. E.; Wittenbrink, M. M. // Veterinary Record: Journal of the British Veterinary Association;11/14/2009, Vol. 165 Issue 20, p594 

    A commercial immunoassay for antibodies to the C6 antigen of Borrelia burgdorferi was evaluated against an IgG in-house ELISA in combination with a Western blot assay to examine 104 samples of serum from 53 healthy Bernese mountain dogs, which were suspected to have a breed predisposition to...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics