TITLE

A Validated Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES) Method to Estimate Free Calcium and Phosphorus in In Vitro Phosphate Binding Study of Eliphos Tablets

AUTHOR(S)
Vallapragada, Venkata Vivekanand; Inti, Gopichand; Sri Ramulu, J.
PUB. DATE
October 2011
SOURCE
American Journal of Analytical Chemistry;Oct2011, Vol. 2 Issue 6, p718
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
An ICP-OES method has been developed to estimate Calcium and Phosphorous in In vitro phosphate binding study of Eliphos Tablets. The method is selective and is capable of detecting calcium and phosphorous in the presence of other trace elements. The method has been validated using RF power of 1500 watts, plasma flow of 15 L/min, Nebuliser flow of 0.8 L/min and plasma view at radial mode for calcium and axial mode for phosphorus. The wavelength was monitored for calcium and phosphorous at 317.933 nm and 213.677 nm respectively. The method has been validated in terms of specificity, precision, linearity, accuracy, limit of quantification and ruggedness. The In vitro binding studies were performed for Eliphos Tablets at eight different phosphate concentrations by incubating at 37.0°C and analysis was performed using the validated method to estimate free calcium and phosphorus. The objective of the study is to provide an alternate In vitro method to estimate the binding capacity of calcium acetate tablets to avoid the expensive in-vivo bio clinical studies.
ACCESSION #
71420950

 

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