Evaluation of growth yield of Spirulina ( Arthrospira) sp. in photoautotrophic, heterotrophic and mixotrophic cultures

Chojnacka, Katarzyna; Zielińska, Agnieszka
February 2012
World Journal of Microbiology & Biotechnology;Feb2012, Vol. 28 Issue 2, p437
Academic Journal
In microbial cultures, both cellular growth rate and yield (defined as the degree of substrate conversion into the biomass) are important. Although effect of culture conditions on growth kinetics has been well documented for various microbial strains, there is almost no literature concerning the effect of environmental conditions on growth equilibrium, expressed as biomass yield coefficients from substrate. The present paper discusses the effect of culture conditions: irradiance (physical substrate) and glucose concentration (chemical substrate) on biomass yield coefficients from two chemical substrates: glucose and nitrate-nitrogen in photoautotrophic, heterotrophic and mixotrophic culture of blue-green alga Spirulina ( Arthrospira) sp. The efficiency of substrates incorporation into the biomass can be precisely determined only if the elemental composition of the biomass is known. The experimental results showed that culture conditions had a substantial influence on biomass yield coefficients (biomass yield from glucose and nitrate-nitrogen). It was found that, the increase of irradiance favoured increase of biomass yield coefficient from both, glucose and nitrate-nitrogen. However, in the case of yield from nitrogen in mixotrophic culture, the effect was opposite. The effect of glucose concentration was different: the higher the initial glucose concentration, the lower the biomass yield coefficients from chemical substrates.

Tags: CELLS -- Growth -- Research;  SPIRULINA;  MICROBIOLOGY -- Cultures & culture media;  MICROALGAE -- Cultures & culture media;  HETEROTROPHIC bacteria;  MICROBIAL growth


Related Articles

  • Taming the smallest predators of the oceans. del Campo, Javier; Not, Fabrice; Forn, Irene; Sieracki, Michael E; Massana, Ramon // ISME Journal: Multidisciplinary Journal of Microbial Ecology;Feb2013, Vol. 7 Issue 2, p351 

    Protists (unicellular eukaryotes) arguably account for most eukaryotic diversity and are central players of the biosphere. Known protist diversity and biology is largely based on cultured strains. Yet, environmental molecular surveys have unveiled entirely novel lineages that, as their...

  • Wastewater treatment to enhance the economic viability of microalgae culture. Pires, J.; Alvim-Ferraz, M.; Martins, F.; Simões, M. // Environmental Science & Pollution Research;Aug2013, Vol. 20 Issue 8, p5096 

    Microalgae culture is still not economically viable and it presents some negative environmental impacts, concerning water, nutrient and energy requirements. In this context, this study aims to review the recent advances on microalgal cultures in wastewaters to enhance their economic viability....

  • Otomycosis due to Filamentous Fungi. Garc�a-Agudo, Lidia; Aznar-Mar�n, Pilar; Gal�n-S�nchez, F�tima; Garc�a-Martos, Pedro; Mar�n-Casanova, Pilar; Rodr�guez-Iglesias, Manuel // Mycopathologia;Oct2011, Vol. 172 Issue 4, p307 

    Otomycosis is common throughout the world but barely studied in Spain. Our objective was to determine the microbiological and epidemiological characteristics of this pathology in Cadiz (Spain) between 2005 and 2010. Samples from patients with suspicion of otomycosis underwent a direct...

  • A Soy-Based Alternative to Traditional Bacterial Nutrient Media. Mosher, Rachel; Crawford, Kyler; Lukowiak, Andrea // American Biology Teacher (National Association of Biology Teache;Jan2009, Vol. 71 Issue 1, p49 

    The article discusses research using soy flour rather than animal-based media for microbiology experiments that support the growth of bacteria by students or researchers. The ethical and environmental concerns of using animal-based products influenced the authors' decision to research this...

  • Study on the effect of mercury (II) chloride as disinfectant on mixed culture. Das, Merina Paul; Rebecca, L. Jeyanthi; Sharmila, S.; Chatterjee, Souvik // Journal of Chemical & Pharmaceutical Research;2012, Vol. 4 Issue 12, p4975 

    Mercuric chloride is very commonly used as disinfectant in biological laboratory. The study was performed to evaluate the effect of mercuric chloride at different concentration on the mixed culture. Contact time was considered as influence parameter, and spread plating-plate counting was used as...

  • A simple laser-based device for simultaneous microbial culture and absorbance measurement. Abrevaya, X.; Cortón, E.; Areso, O.; Mauas, P. // Instruments & Experimental Techniques;Jul2013, Vol. 56 Issue 4, p472 

    In this work we present a device specifically designed to study microbial growth with several applications related to environmental microbiology and other areas of research as astrobiology. The Automated Measuring and Cultivation device (AMC-d) enables semi-continuous absorbance measurements...

  • Growth-associated biosynthesis of astaxanthin in heterotrophic Chlorella zofingiensis (Chlorophyta). J. Peng // World Journal of Microbiology & Biotechnology;Sep2008, Vol. 24 Issue 9, p1915 

    Abstract  The green microalga Chlorella zofingiensis can produce the ketocarotenoid astaxanthin under heterotrophic culture conditions. Here we report the growth-associated biosynthesis of astaxanthin in this biotechnologically important alga. With glucose as sole carbon and energy source,...

  • Kinetics of styrene biodegradation by Pseudomonas sp. E-93486. Gąszczak, Agnieszka; Bartelmus, Grażyna; Greń, Izabela // Applied Microbiology & Biotechnology;Jan2012, Vol. 93 Issue 2, p565 

    The research into kinetics of styrene biodegradation by bacterial strain Pseudomonas sp. E-93486 coming from VTT Culture Collection (Finland) was presented in this work. Microbial growth tests in the presence of styrene as the sole carbon and energy source were performed both in batch and...

  • Evaluation of guar gum derivatives as gelling agents for microbial culture media. Gangotri, Waikhom; Jain-Raina, Ruchi; Babbar, Shashi // World Journal of Microbiology & Biotechnology;May2012, Vol. 28 Issue 5, p2279 

    Guar gum, a galactomannan, has been reported to be an inexpensive substitute of agar for microbial culture media. However, its use is restricted probably because of (1) its highly viscous nature even at high temperatures, making dispensing of the media to Petri plates difficult and (2) lesser...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics