Characterization of Mouse tGolgin-1 (Golgin-245/trans-Golgi p230/256 kD Golgin) and Its Upregulation during Oligodendrocyte Development

Cowan, David A.; Gay, Denise; Bieler, Bert M.; Zhao, Huizhen; Yoshino, Atsuko; Davis, James G.; Tomayko, Mary M.; Murali, Ramachandran; Greene, Mark I.; Marks, Michael S.
July 2002
DNA & Cell Biology;Jul2002, Vol. 21 Issue 7, p505
Academic Journal
As part of an effort to identify gene products that are differentially regulated during oligodendrocyte development, we isolated a mouse cDNA that encodes tGolgin-1, a homolog of the human protein known as golgin-245, trans-golgi p230, or 256 kD golgin. Human tGolgin-1 is the target of autoantibodies in patients with Sj�gren's syndrome, and is thought to be involved in vesicular transport processes at the trans-Golgi network. Sequencing of cDNAs and EST clones comprising the full-length tGolgin-1 transcript predict marked homology with the amino- and carboxy-terminal regions of the human protein, but more limited homology within the central predicted coiled-coil region. Epitope tagged, truncated forms of mouse tGolgin-1, like those of its human homolog, were localized at steady state to the Golgi/trans-Golgi network in transfected cells. The tGolgin-1 message was expressed in all tissues examined, but was highly upregulated in oligodendrocyte precursors at a stage just prior to myelination. This expression pattern suggests that tGolgin-1 may play a role in specialized transport processes associated with maturation and/or differentiation of oligodendrocyte precursors.


Related Articles

  • Researchers develop model for better testing, targeting of MPNST.  // Medical Device Daily;5/24/2013, Vol. 17 Issue 101, Special section p2 

    The article focuses on a new mouse model for malignant peripheral nerve sheath tumors (MPNST) developed by researchers from the Masonic Cancer Center of the University of Minnesota. The model allows the researchers to discover new genes and gene pathways driving this kind of cancer. An unbiased...

  • Gene Expression Mapping of Histone Deacetylases and Co-factors, and Correlation with Survival Time and 1H-HRMAS Metabolomic Profile in Human Gliomas. Dali-Youcef, Nassim; Froelich, Sébastien; Moussallieh, François-Marie; Chibbaro, Salvatore; Noël, Georges; Namer, Izzie J.; Heikkinen, Sami; Auwerx, Johan // Scientific Reports;3/20/2015, p9087 

    Primary brain tumors are presently classified based on imaging and histopathological techniques, which remains unsatisfaying. We profiled here by quantitative real time PCR (qRT-PCR) the transcripts of eighteen histone deacetylases (HDACs) and a subset of transcriptional co-factors in...

  • Identification of a Gene Regulatory Network Necessary for the Initiation of Oligodendrocyte Differentiation. Swiss, Victoria A.; Tung Nguyen; Dugas, Jason; Ibrahim, Adiljan; Barres, Ben; Androulakis, Ioannis P.; Casaccia, Patrizia // PLoS ONE;2011, Vol. 6 Issue 4, p1 

    Differentiation of oligodendrocyte progenitor cells (OPCs) into mature oligodendrocytes requires extensive changes in gene expression, which are partly mediated by post-translational modifications of nucleosomal histones. An essential modification for oligodendrocyte differentiation is the...

  • Overexpression of miR-98 inhibits cell invasion in glioma cell lines via downregulation of IKKε. FAN, Y.-H.; YE, M.-H.; WU, L.; LV, S-G; WU, M.-J.; XIAO, B.; LIAO, C.-C.; JI, Q.-K.; CHAI, Y.; ZHU, X.-G. // European Review for Medical & Pharmacological Sciences;OCt2015, Vol. 19 Issue 19, p3593 

    OBJECTIVE: MicroRNAs (miR-NAs) function as negative regulators for the expression of genes involved in cancer metastasis. The aim of this study was to investigate the potential role of miR-98 in gliomas and validate its regulatory mechanism. PATIENTS AND METHODS: Cell viability assays are used...

  • Clinic forms center devoted to tumors of nervous system. Santiago, Raquel // Crain's Cleveland Business;7/24/95, Vol. 16 Issue 30, p9 

    Reports on the Cleveland Clinic Foundation's establishment of the Brain Tumor and Neuro-Oncology Center in Cleveland, Ohio to consolidate research and clinical efforts in studying tumors of the nervous system. Appointment of Gene Barnett as director; Cutting edge studies at the center.

  • Pathology and molecular genetics of astrocytic gliomas. Reifenberger, Guido; Collins, Vincent Peter // Journal of Molecular Medicine;Oct2004, Vol. 82 Issue 10, p656 

    Astrocytic gliomas are the most common primary brain tumours. Here we summarize the characteristic neuropathological features of the different types of astrocytic neoplasms according to the World Health Organization classification of tumours of the nervous system. In addition, we report on the...

  • Automatized assessment of 1p36-19q13 status in gliomas by interphase FISH assay on touch imprints of frozen tumours. Belaud-Rotureau, Marc-Antoine; Meunier, Nelly; Eimer, Sandrine; Vital, Anne; Loiseau, Hugues; Merlio, Jean-Philippe // Acta Neuropathologica;Mar2006, Vol. 111 Issue 3, p255 

    Molecular genetic analyses have demonstrated that combined losses in 1p36 and 19q13 were associated with a good response to treatment and a higher survival rates in oligodendrogliomas (O). The presence of such deletions in a subset of mixed oligoastrocytomas (OA) also suggests that 1p–19q...

  • A cluster of genes located in 1p36 are down-regulated in neuroblastomas with poor prognosis, but not due to CpG island methylation. Caren, Helena; Ejeskar, Katarina; Fransson, Susanne; Hesson, Luke; Latif, Farida; Sjoberg, Rose-Marie; Krona, Cecilia; Martinsson, Tommy // Molecular Cancer;2005, Vol. 4, p10 

    Background: A common feature of neuroblastoma tumours are partial deletions of the short arm of chromosome 1 (1p-deletions). This is indicative of a neuroblastoma tumour suppressor gene being located in the region. Several groups including our have been studying candidate neuroblastoma genes in...

  • Medulloblastoma: a potpourri of distinct entities. Pfister, Stefan // Acta Neuropathologica;Apr2012, Vol. 123 Issue 4, p463 

    An introduction is presented in which the author discusses various reports within the issue on topics including the classification of World Health Organization (WHO) to central nervous system (CNS) tumors, medulloblastoma gene expression, and sonic hedgehog (SHH)-driven medulloblastoma.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics