Effect of chitinase and thaumatin on mycelial growth of five sorghum [Sorghum bicolor (L.) Moench] grain molding fungi under in vitro conditions

Prom, Louis K.; Egilla, Jonathan
June 2011
Journal of Tropical Agriculture;2011, Vol. 49 Issue 1/2, p88
Academic Journal
The effects of various concentrations of chitinase, thaumatin, and their mixtures on radial mycelial growth of five grain molding fungi were studied. When compared with the control, chitinase and chitinase+thaumatin mixtures markedly reduced mycelial growth of the fungal species studied, except for Fusarium semitectum. Reductions in fungal radial mycelial growth of 65.4% for Bipolaris sp. and 61.7% for Colletotrichum graminicola were noted when these fungi were grown on half-strength-potato dextrose agar amended with chitinase. A mixture of chitinase at 50 ml L-1+thaumatin at 37.5 mg L-1 reduced mycelial growth of Bipolaris sp. by 61.7%, C. graminicola by 56.2%, and Curvularia lunata by 44.9% when compared with the control. The levels of thaumatin used in this study were less effective in inhibiting mycelial growth, except for F. semitectum. This study for the first time showed the suppression of mycelial growth in vitro of F. thapsinum, Bipolaris sp., and C. graminicola isolated from sorghum grain.


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