Molecular Diagnostics of Human Papillomavirus

Arney, Ashley; Bennett, Katie M.
September 2010
Laboratory Medicine;Sep2010, Vol. 41 Issue 9, p523
Academic Journal
Purpose: Human papillomavirus (HPV) infection is the most common sexually transmitted infection in the United States. This review summarizes the molecular testing methods currently in use for the detection and genotyping of HPV DNA and discusses potential future approaches. Summary: There are more than 100 different types of HPV, which are separated into low- and high-risk categories, depending on clinical presentation. Low-risk types can cause warts, while high-risk types are associated with cervical cancer. The available methods for HPV detection and genotyping include both target and signal amplification techniques. Conclusion: Molecular diagnostic methods allow for the identification of the many unique types of high-risk HPV, which can lead to better diagnoses and treatment plans for patients. Use of these diagnostic techniques requires consideration of the consensus guidelines for management of women with normal and abnormal cervical screens, as well as consideration of assay clinical sensitivity and diagnostic utility.


Related Articles

  • HPV: A LOOK INTO NEW METHODS FOR HIGH-RISK TESTING. Reynolds, Jordan P. // MLO: Medical Laboratory Observer;Jul2011, Vol. 43 Issue 7, p10 

    The article discusses various screening test methods used for human papillomavirus (HPV) infection. The methods discussed include the nucleic-acid hybridization assay probe set called Digene Hybrid Capture 2, polymerase chain reaction (PCR) based DNA-detection system called Cobas 4800, and...

  • Prevención secundaria (detección) del cáncer cervicouterino.  // Ginecologia y Obstetricia de Mexico;dic2011, Vol. 79 Issue 12, p808 

    No abstract available.

  • Liquid based cytology. Masenya, M. // Obstetrics & Gynaecology Forum;Aug2011, Vol. 21 Issue 3, p9 

    The human papilloma virus has been shown to play a significant aetiological role in invasive cervical cancer. The changes caused by human papilloma virus can be evaluated using Pap smear. The discovery of Pap smears has led to an unprecedented dramatic decline in the incidence of cervical cancer...

  • The Test You're Probably NOT Getting.  // Good Housekeeping;Apr2010, Vol. 250 Issue 4, p55 

    The article focuses on screening for cervical cancer in women. The author notes that a more sensitive way to screen for cervical cancer is the human papillomavirus (HPV) test. The effectiveness of the test is discussed and a comparison with the traditional Pap test is given. Guidelines for both...

  • ORGANISATIONAL ASPECTS OF SUCCESSFUL SCREENING PROGRAMMES IN DEVELOPED COUNTRIES: THE ENGLISH MODEL. Kitchener, Henry // Infectious Diseases in Obstetrics & Gynecology;2006, Vol. 2006, p6 

    Assessing benefits, risks, and costs of cancer screening In planning a cancer screening programme, each country will develop guidelines based on the best available estimates of costs, benefits, and risks. Guidelines can be reviewed in light of emerging evidence as well as new diagnostic and...

  • The role of human papillomavirus testing in cervical cancer screening. Morasse, Lauren; Davidov, Adi; Castellanos, Mario R. // JAAPA: Journal of the American Academy of Physician Assistants (;Nov2009, Vol. 22 Issue 11, p20 

    The article evaluates the cervical cancer screening guidelines with a focus on the role the human papillomavirus (HPV) testing. It states that the two screening methods include the conventional Pap smear where a sample of cervical cells are placed directly on a slide and the liquid-based...

  • HPV testing superior to Pap smear, safe to extend screening to once every 3 years. Markman, Maurie // Infectious Disease News;Jun2011, Vol. 24 Issue 6, p29 

    The article reports on a study which showed that human papilloma virus (HPV) testing is more accurate than Pap smear for determining cervical cancer risk.

  • Peace of mind after the Pap test? Marcus, Mary Brophy // U.S. News & World Report;03/05/2001, Vol. 130 Issue 9, p63 

    Reports on a research relating to tests for cervical cancer. The study in the February 2001 issue of 'Journal of the National Cancer Institute' which suggests a noninvasive test for human papillomavirus (HPV) as a follow-up to a Pap smear; The yearly percentage of women with borderline abnormal...

  • Significant Progression of Uterine Cervical Epithelial Lesion Accompanied by Marked Increase in 3q26 Gene Amplification. Verri, Annalisa; Jalali, G. Reza; Cecchini, Giancarlo; Diani, Stefano; Dorji, Tshering; Kilpatrick, Michael W.; Grazioli, Vittorio; Walat, Robert J. // Laboratory Medicine;Mar2011, Vol. 42 Issue 3, p134 

    Clinical History Patient: A 35-year-old Caucasian female Chief Complaint: Abnormal cervical smear History of Present Illness and Principal Laboratory Findings: A ThinPrep Pap Test reported atypical squamous cells of uncertain significance in November 2008. The patient tested positive for human...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics