TITLE

Flow Cytometric Immunophenotypic Assessment of T-Cell Clonality by Vβ Repertoire Analysis

AUTHOR(S)
Tembhare, Prashant; Yuan, Constance M.; Liqiang Xi; Morris, John C.; Liewehr, David; Venzon, David; Janik, John E.; Raffeld, Mark; Stetler-Stevenson, Maryalice
PUB. DATE
June 2011
SOURCE
American Journal of Clinical Pathology;Jun2011, Vol. 135 Issue 6, p890
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Flow cytometric T-cell receptor (TCR)-Vβ repertoire analysis (TCR-Vβ-R) is a sensitive method for detection of T-cell clonality; however, no uniform approach exists to define clonality in neoplastic T cells. TCR-Vβ-R was evaluated in patients with a diagnosis of T-cell neoplasia in initial diagnostic specimens from 41 patients and for minimal residual disease (MRD) monitoring in 61 sequential samples from 14 patients with mature T-cell neoplasia. Gating strategies and criteria for detection of T-cell clonality were determined. In all 41 initial specimens, T-cell clonality was demonstrated via TCR-Vβ-R. The frequency of Vβ usage was consistent with random neoplastic transformation of TCR-Vβ subsets. MRD was successfully detected in follow-up samples from all 14 patients evaluated, Furthermore, MRD after therapy was quantitated in 48 peripheral blood specimens. TCR-Vβ-R analysis is a sensitive method for detection of T-cell clonality and is useful for diagnosis and MRD detection in multiple specimen types.
ACCESSION #
70108854

 

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