TITLE

NUCLEOTIDE SEQUENCING AND PCR-SSCP OF MX1 GENE IN CHICKEN

AUTHOR(S)
Chinmoy Mishra; Dharmeswar Das; Kumar, Pushpendra; Khanna, Kuldeep; Singh, Ajit Pratap; Dayal, Shanker; Bhattacharya, Tarun Kumar; Bhushan, Bharat; Sharma, Arjava
PUB. DATE
December 2011
SOURCE
Indian Journal of Animal Research;Dec2011, Vol. 45 Issue 4, p276
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Mx1 is an interferon induced gene which inhibits the proliferation of single stranded RNA viruses. The size of Mx1 gene of chicken (Gallus gallus) is 21289 bp, having 14 exons and the coding sequence is 2118 bp long. Differential antiviral activity of Mx protein has been proved in various species including chicken. Polymerase Chain Reaction and subsequent Single Stranded Conformation Polymorphism (PCR-SSCP) analysis of 284 bp fragment of this gene was conducted to screen 153 birds of Aseel breed of chicken. Three genotypes viz. AB (0.74), BC (0.12) and AC (0.14) with three different alleles viz. A (0.44), B (0.43) and C (0.13) were identified. The homozygous genotypes i.e. AA, BB and CC could not be found in this population. Nucleotide sequencing of different alleles was done and submitted to the NCBI GenBank data (Acc. No.-GQ912704, GQ912705 and GQ912706). Nucleotide sequences were aligned with that of the corresponding Mx1 gene sequences of different breeds of chicken using DNASTAR Lasergene Software which revealed variations at five positions (91st, 107th, 113th, 194th and 231st). There is an urgent need of wide search for detection of polymorphism in Mx1 gene of different breeds of chicken and its possible association with the traits of economic importance.
ACCESSION #
70107364

 

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