Necessity of Bilateral Bone Marrow Biopsies for Ancillary Cytogenetic Studies in the Pediatric Population

Grimm, Kate E.; Chen, Connie; Weiss, Lawrence M.
December 2010
American Journal of Clinical Pathology;Dec2010, Vol. 134 Issue 6, p982
Academic Journal
The need for bilateral pediatric bone marrow biopsies for cytogenetic and fluorescent in situ hybridization (FISH) studies has not been clearly delineated. We retrospectively identified 166 pediatric bilateral bone marrow biopsy specimens obtained from patients with a variety of clinical diagnoses, including solid tumors, lymphoma, leukemia, and other hematologic conditions. The cases included all pediatric bilateral bone marrow biopsies performed at our hospital spanning the years of 1992 to 2008. Agreement of FISH and classical cytogenetic results between the 2 sides was assessed. Of a total of 166 bilateral cases, 2 cases showed disagreement (1.2%), both from patients with solid tumors. One case was a rhabdomyosarcoma, in which FISH only was performed; the second was a neuroblastoma in which FISH and cytogenetics were performed (both FISH and classical cytogenetic results disagreed). The remainder of the cases showed complete agreement between the 2 sides (total 98.8%). We conclude that it is usually not necessary to perform bilateral bone marrow biopsies for FISH and cytogenetics in the pediatric population outside of the setting of solid tumor staging.


Related Articles

  • Contribution of bone marrow-derived endothelial cells to human tumor vasculature. Peters, Brock A.; Diaz Jr., Luis A.; Polyak, Kornelia; Meszler, Leslie; Romans, Kathy; Guinan, Eva C.; Antin, Joseph H.; Myerson, David; Hamilton, Stanley R.; Vogelstein, Bert; Kinzler, Kenneth W.; Lengauer, Christoph // Nature Medicine;Mar2005, Vol. 11 Issue 3, p261 

    It has been shown that bone marrow-derived stem cells can form a major fraction of the tumor endothelium in mouse tumors. To determine the role of such cells in human tumor angiogenesis, we studied six individuals who developed cancers after bone marrow transplantation with donor cells derived...

  • Myelodysplastic syndrome in children: differentiation from acute myeloid leukemia with a low blast count. Chan, G C F; Wang, W C; Raimondi, S C; Behm, F G; Krance, R A; Chen, G; Freiberg, A; Ingram, L; Butler, D; Head, D R // Leukemia (08876924);Feb97, Vol. 11 Issue 2, p206 

    Bone marrow from six patients with acute myeloid leukemia (AML) and t(8;21) (q22;q22) or a variant t(8;13;21) was studied by simultaneous analysis of cell morphology and karyotype. Combination of May -- Gru ünwald -- Giemsa (MGG) and fluor-escence in situ hybridization (FISH) using probes...

  • Fluorescence in situ hybridization analysis of chromosome aberrations in 60 Chinese patients with multiple myeloma. Gao, Xiao; Li, Chunming; Zhang, Run; Yang, Ruifang; Qu, Xiaoyan; Qiu, Hairong; Xu, Jiaren; Lu, Hua; Li, Jianyong; Chen, Lijuan // Medical Oncology;Sep2012, Vol. 29 Issue 3, p2200 

    Conventional cytogenetic analysis is often hampered owing to the low mitotic index of multiple myeloma (MM) cells in bone marrow samples of MM. Interphase fluorescence in situ hybridization (I-FISH) analysis combined with magnetic-activated cell sorting (MACS) has substantially enhanced the...

  • Chemically-Induced Cancers Do Not Originate from Bone Marrow-Derived Cells. Hui Lin; Liang Hu; Leilei Chen; Hong Yu; Qi Wang; Ping Chen; Xiao-Tong Hu; Xiu-Jun Cai; Xin-Yuan Guan // PLoS ONE;Jan2012, Vol. 7 Issue 1, p1 

    Background: The identification and characterization of cancer stem cells (CSCs) is imperative to understanding the mechanism of cancer pathogenesis. Growing evidence suggests that CSCs play critical roles in the development and progression of cancer. However, controversy exists as to whether...

  • Early detection of relapse and evaluation of treatment for mixed chimerism using fluorescence in situ hybridization following allogeneic hematopoietic cell transplant for hematological malignancies. Tamura, S.; Saheki, K.; Takatsuka, H.; Wada, H.; Fujimori, Y.; Okamoto, T.; Takemoto, Y.; Hashimoto-Tamaoki, T.; Furuyama, J. i.; Kakishita, E. // Annals of Hematology;Nov2000, Vol. 79 Issue 11, p622 

    In order to detect chimerism, fluorescence in situ hybridization (FISH) and cytogenetic analyses were performed on bone marrow cells from 47 patients with hematological malignancies following allogeneic hematopoietic cell transplant (HCT). The dual-color XY, major Bcr-Abl (M-Bcr-Abl), and...

  • Microarray-based genomic profiling and in situ hybridization on fibrotic bone marrow biopsies for the identification of numerical chromosomal abnormalities in myelodysplastic syndrome. Stevens-Kroef, Marian JPL; Hebeda, Konnie M.; Verwiel, Eugène T.; Kamping, Eveline J.; van Cleef, Patricia H.; Kuiper, Roland P.; Groenen, Patricia JTA // Molecular Cytogenetics (17558166);2015, Vol. 8 Issue 1, p1 

    Background: Myelodysplastic syndromes (MDS) are a heterogeneous group of clonal hematological malignancies. In MDS patients with a fibrotic bone marrow the aspiration of cells often fails (dry-tap), which hampers standard karyotyping. Obtaining genetic data from these fibrotic marrows is...

  • The role of cytogenetics in myeloma. Zhan, F.; Sawyer, J.; Tricot, G. // Leukemia (08876924);Sep2006, Vol. 20 Issue 9, p1484 

    The article analyzes the role of cytogenetics in myeloma, in leukemia research. Multiple myeloma is a slow progressing disease caused by the death of mature B cells of bone marrow. Active stage is preceded by dormant phase. The different methods of treatment like deletion of chromozome 13,...

  • Cytogenetics of Multiple Myeloma. Trčić, Ružica Lasan; Skelin, Ika Kardum; Šušterčić, Dunja; Planinc-Peraica, Ana; Ajduković, Radmila; Hariš, Višnja; Kušec, Rajko; Begović, Davor // Collegium Antropologicum;Mar2010, Vol. 34 Issue 1, p41 

    Great studies of multiple myeloma (MM) strongly suggested that specific chromosomal changes are of prognostic significance in patients with MM¹. We have performed cytogenetic analysis and recently fluorescent in situ hybridization (FISH) on 43 cases of MM. Clonal chromosomal changes were...

  • Can Reflex UroVysion fluorescence in situ hybridization predict tumor recurrence during follow-up? Kurien, Annamma; Thomas, Joseph // Indian Journal of Urology;Jul-Sep2007, Vol. 23 Issue 3, p333 

    The primary aim of this study was to demonstrate the usefulness of multitarget fluorescence in situ hybridization (FISH UroVysion) testing in the early detection of recurrent urothelial carcinoma (UC). The study population comprised patients with bladder tumor on follow-up, who had negative or...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics