Identification of Two Borderline Oxacillin-Resistant Strains of Staphylococcus aureus From Routine Nares Swab Specimens by One of Three Chromogenic Agars Evaluated for the Detection of MRSA

Buchan, Blake W.; Ledeboer, Nathan A.
December 2010
American Journal of Clinical Pathology;Dec2010, Vol. 134 Issue 6, p921
Academic Journal
Methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of nosocomial infections that result in extended hospital stays and increased mortality. Therefore, rapid, cost-effective techniques for surveillance and detection of MRSA are critical to the containment and prevention of the spread of MRSA within the health care environment. We examined the ability of 3 chromogenic media (Spectra MRSA, Remel, Lenexa, KS; MRSA Select, Bio-Rad, Redmond, WA; and ChromID MRSA, bioMerieux, Marcy l'Etoile, France) to detect MRSA from routine surveillance specimens following 18, 24, and 48 hours of incubation. Our results indicate that detection of MRSA using all 3 chromogenic media is optimal following 24 hours of incubation. Early examination reduced sensitivity, while extended incubation reduced specificity. In addition, Spectra MRSA identified 2 borderline oxacillin-resistant strains of S aureus that were not detected by the other 2 chromogenic agars evaluated. These strains demonstrate increased basal and inducible resistance to β-lactam antibiotics.


Related Articles

  • Comparison of three chromogenic media and enrichment broth media for the detection of methicillin-resistant Staphylococcus aureus from mucocutaneous screening specimens. Nonhoff, C.; Denis, O.; Brenner, A.; Buidin, P.; Legros, N.; Thiroux, C.; Dramaix, M.; Struelens, M. J. // European Journal of Clinical Microbiology & Infectious Diseases;Apr2009, Vol. 28 Issue 4, p363 

    This study compares the performance of three chromogenic culture agar plates, chromID MRSA, MRSA-Screen and MRSA-Select, by challenging with a collection of Staphylococcus aureus strains and screening samples obtained from hospitalised patients. All chromogenic media showed excellent sensitivity...

  • An admission of death. Robins, Jane // New Statesman;09/26/97, Vol. 126 Issue 4353, p46 

    Focuses on methicillin-resistant staphylococcus aureus (MRSA). Deaths in hospitals resulting from MRSA; Portal of entry of damage to the integrity of the skin; Doctors and nurses' ignorance of precautionary measures.

  • Frequency of Mupirocin Resistant Staphylococcus aureus Strains Isolated From Nasal Carriers in Hospital Patients in Kermanshah. Mohajeri, Parviz; Gholamine, Babak; Rezaei, Mansour; Khamisabadi, Yazdan // Jundishapur Journal of Microbiology;2012, Vol. 5 Issue 4, p560 

    Background: Staphylococcus aureus is a major nosocomial pathogen world wide. Mupirocin plays a crucial role in strategies designed to control outbreaks of S. aureus. Objectives: The aim of this study was to determine the frequency of mupirocin resistance in S. aureus strains isolated from nasal...

  • An uncommon presentation for a severe invasive infection due to methicillin-resistant Staphylococcus aureus clone USA300 in Italy: a case report. Valentini, Piero; Parisi, Gabriella; Monaco, Monica; Crea, Francesca; Spanu, Teresa; Ranno, Orazio; Tronci, Mirella; Pantosti, Annalisa // Annals of Clinical Microbiology & Antimicrobials;2008, Vol. 7, Special section p1 

    Background: Methicillin resistant Staphylococcus aureus (MRSA) has been considered for many years a typical nosocomial pathogen. Recently MRSA has emerged as a frequent cause of infections in the community. More commonly, community-acquired (CA)-MRSA is a cause of infections of the skin and...

  • Hospital-acquired MRSA increased active infection rate. Cox, Amber; Schoen, John; Shafer, Emily // Infectious Disease News;Oct2013, Vol. 26 Issue 10, p22 

    The article discusses research on the association between methicillin-resistant Staphylococcus aureus (MRSA) during hospitalization and increased risk for developing an active infection, presented at the Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC) in 2013.

  • Outbreak of MRSA among newborns and mothers likely hospital-acquired. Foster, Melissa // Infectious Diseases in Children;Apr2010, Vol. 23 Issue 4, p15 

    The article discusses a study on methicillin-resistant Staphylococcus aureus (MRSA) among newborns and mothers which was likely acquired at hospital, presented at the Conference on Healthcare-Associated Infections in Atlanta, Georgia, in March 2010.

  • Methicillin-resistant Staphylococcus aureus, Western Australia. Dailey, Lynne; Coombs, Geoffrey W.; O'Brien, Frances G.; Pearman, John W.; Christiansen, Keryn; Grubb, Warren B.; Riley, Thomas V. // Emerging Infectious Diseases;Oct2005, Vol. 11 Issue 10, p1584 

    Methicillin-resistant Staphylococcus aureus (MRSA) continues to be a notable cause of hospital-acquired infections. A statewide screening and control policy was implemented in Western Australia (WA) after an outbreak of epidemic MRSA in a Perth hospital in 1982, We report on statutory...

  • Nasal Colonization rate of Staphylococcus aureus strains among Health Care Service Employee's of Teaching University Hospitals in Yazd. Khalili, Mohammad Bagher; Sharifi-Yazdi, Mohammad Kazem; Dargahi, Hosein; Sadeghian, Hosein Ali // Acta Medica Iranica;2009, Vol. 47 Issue 4, p315 

    This study was carried out to find the extent of staphylococcal carriages including Methicillin resistant Staphylococcus aureus MRSA in employee's of teaching university hospitals in Yazd. Nasal swabs of 742 employees in four different medical teaching hospitals in Yazd were collected, and...

  • Evaluation of S. aureus ID, a Chromogenic Agar Medium for the Detection of Staphylococcus aureus. Diederen, B.; Leest, C.; Duijn, I.; Willemse, P.; Keulen, P.; Kluytmans, J. // Infection;Apr2006, Vol. 34 Issue 2, p95 

    Background: Screening for staphylococci among various patient populations has become important for appropriate therapeutic management and for control of nosocomial infections. The purpose of this study is to evaluate the in vitro sensitivity and specificity of a chromogenic agar medium, S....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics