TITLE

EVALUATION OF PCR TECHNIQUES FOR DETECTION AND DIFFERENTIATION OF CANINE ADENOVIRUSES IN FAECAL SAMPLES IN SHIRAZ, IRAN

AUTHOR(S)
MOHAMMADI, A.; MASOUDIAN, M.; NEMATI, Y.
PUB. DATE
December 2011
SOURCE
Bulgarian Journal of Veterinary Medicine;Dec2011, Vol. 14 Issue 4, p247
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Canine adenovirus type 1 and 2 (CAV-1 & CAV-2) are causative agents of infectious canine hepatitis and infectious canine laryngotracheitis, respectively. Both viruses are shed in faeces and urine of the infected or recovered dogs. A total of 75 faecal samples were collected from diarrheic and nondiarrheic dogs referred to the small animal clinics of the Veterinary School, Shiraz University, Iran. Polymerase chain reaction was performed using separately three sets of primers (HA1/HA2, CAVVP1/ CAV-VP2/, and CAV-F1/CAV-R1). The results showed 28 positive samples, including 16 CAV-1 and 12 CAV-2. According to the study, the molecular diagnosis of CAV in faeces could be performed with better results using the primers CAV-VP1/ CAV-VP2 and CAV-F1/ CAV-R1.
ACCESSION #
69748795

 

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