TITLE

Ultrastructural studies on actin-like filament in mung bean mitochondria and its potential functional significance

AUTHOR(S)
Yih-Shan Lo; Lin-June Hsiao; Wann-Neng Jane; Ning Cheng; Hwa Dai
PUB. DATE
October 2011
SOURCE
Botanical Studies;2011, Vol. 52 Issue 4, p445
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
This document reports that actin-like filaments, about 5 nm in width, were found in mung bean mitochondria after negative staining of mitoplast. The 5 nm filaments, composed of globular proteins, were visualized beneath the intramembranous particles by freeze-fracture electron microscope. Freeze-fracture immuno- labeling against anti-actin antibody suggested that actin-like filaments attached to the protoplasmic surface of mitochondrial inner membrane. These filaments were also found in the matrix and underneath of outer membrane. The presence of actin-like protein in mitochondria was also substantiated by immunolocalization in situ. Interestingly, some 5 nm filamentous bundles were often found between mitochondrion and its "bud". It is likely that these images represent an undiscovered novel plant mitochondrial budding process which may involve some 5 nm filamentous structure in separating nascent mitochondrion to its mother mitochondrion. Treatment of cultured tobacco cells with F-actin depolymerization reagent, Latrunculin B (Lat B) might affect the shapes of mitochondria. Taken together, this study suggests that some 5 nm filaments, probably F-actin, may be localized in mitochondria and play a role in mitochondrial propagation and mitochondrial shaping.
ACCESSION #
69720870

 

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