TITLE

Mycobased biosynthesis of Silver nanoparticles and Studies of its Synergistic antibacterial activity combined with Cefazolin antibiotic against selected organisms

AUTHOR(S)
Singh, Prashant; Kumar, Ritesh; Raja, R. Balaji; Kalaichelvan, P. T.
PUB. DATE
August 2011
SOURCE
Journal of Applied Sciences Research;Aug2011, Vol. 7 Issue 8, p1412
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Silver nanoparticles were synthesized biologically from non pathogenic fungus and its synergistic antibacterial activity with the antibiotic cefazolin was studied in detail. The nanoparticles were characterized using UV-Vis spectroscopy and Transmission Electron Microscopy (TEM). In the UV - Vis spectrum the peak at 420nm confirms the synthesis of silver nanoparticles and from TEM studies silver nanoparticles with different shapes and sizes were observed. The size of the silver nanoparticles was observed in the range of 30-50 nm with spherical shape. The synthesis of nanoparticles was rapid, stable for several months and involves purely 'green synthesis' which does not involve any toxic chemicals and by products. The antibacterial activity for silver nanoparticles, antibiotic and antibiotic conjugated with silver nanoparticles was assessed using the standard broth dilution method and pour plate technique for determining the minimum inhibitory concentration (MIC). Organisms used for this study are Escherichia coli (Gram negative) and Staphylococcus aureus (Gram positive). The antibacterial effect of the antibiotic and the silver nanoparticles was found separately and compared with that of combined effect of antibiotic and silver nanoparticles and the combination of antibiotic with silver nanoparticles was found to be very effective compared to its individual effect. The current study opens light to a novel drug with broad spectrum antibacterial activity.
ACCESSION #
69681722

 

Related Articles

Share

Read the Article

Courtesy of NEW JERSEY STATE LIBRARY

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics