TITLE

Leishmania major in Tatera indica in Estahban, Southern Iran: Microscopy, culture, isoenzyme and PCR

AUTHOR(S)
Mehrabani, Davood; Motazedian, Mohammad Hossein; Asgari, Qasem; Hatam, Gholam Reza; Owji, Seyed Mohammad; Oryan, Ahmad
PUB. DATE
July 2011
SOURCE
Pakistan Journal of Medical Sciences;Jul2011, Vol. 27 Issue 4, p734
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Objectives: This study identifies the infected rodent hosts to Leishmania major in Estahban town, southern Iran during 2004-2005. Methodology: The rodents were caught alive from April 2004 to April 2005 in Estahban town, south of Iran and examined for any skin lesion. An impression was provided from the tissues of feet, tail, ears and any patent lesion, stained with Giemsa and studied microscopically for the presence of amastigotes. All samplings were cultured at 25°C in rabbit blood agar and considered negative if no promastigotes were visible during a two months period. The parasites from any positive culture were cryopreserved in liquid nitrogen pending their identification in PCR and isoenzyme electrophoresis. The femoral bones were histologically and ultrastrucrurally studied. Results: Among 13 captured rodents, 8 were Tatera indica (5 male and 3 female Indian gerbils) and 5 were Rattus rattus (3 males and 2 females). Just one female T. indica was smear-positive for amastigotes in Mohmmad Abad village. This rodent was also found culture positive for leishmanial infection which was confirmed by PCR and enzyme electrophoresis. At histological and ultrastructural levels, many clusters of amastigotes were noticed in the foamy macrophages of the femoral bone bone marrow. Conclusion: T. indica was found for the first time in the area and can be one of the rodents to be a potential reservoir host of L. major. It was also shown that femoral bone marrow was the tissue of choice to confirm the presence of macrophages containing the amastigote form of the parasite.
ACCESSION #
69617709

 

Related Articles

  • Leishmania major in Tatera indica in Fasa, Southern Iran: Microscopy, Culture, Isoenzyme, PCR and Morphologic Study. Mehrabani, D.; Motazedian, M. H.; Hatam, G. R.; Asgari, Q.; Owji, S. M.; Oryan, A. // Asian Journal of Animal & Veterinary Advances;Mar2011, Vol. 6 Issue 3, p255 

    This study was undertaken to identify rodents infected with Leishmania in Fasa town, Fars Province, Southern Iran. From April 2004 to April 2005, a total of 17 rodents were collected in live traps in the area. Microscopical examination of stained tissue smears and the culture of tissue samples,...

  • Identification of Species Causing Cutaneous Leishmaniasis by PCR in Chahbahar, Iran. Soleimanpoor, Hosein; Dabirzadeh, Mansour; Fooladi, Bahman // Medical Laboratory Journal;Mar/Apr2016, Vol. 10 Issue 2, p46 

    Background and Objective: Chabahar is in Southern Iran located near the Iran- Pakistan border. Since leishmaniasis is an emerging disease in this region, this study aimed to diagnose the disease and identify different species of Leishmania parasite in the patients referred to the central...

  • A2 gene of Old World cutaneous Leishmania is a single highly conserved functional gene. Garin, Yves J. F.; Meneceur, Pascale; Pratlong, Francine; Dedet, Jean-Pierre; Derouin, Francis; Frédéric Lorenzo // BMC Infectious Diseases;2005, Vol. 5 Issue 1, p1 

    Background: Leishmaniases are among the most proteiform parasitic infections in humans ranging from unapparent to cutaneous, mucocutaneous or visceral diseases. The various clinical issues depend on complex and still poorly understood mechanisms where both host and parasite factors are...

  • Persistence of Leishmania Parasites in Scars after Clinical Cure of American Cutaneous Leishmaniasis: Is There a Sterile Cure? Mendonça, Mitzi G.; de Brito, Maria E. F; Rodrigues, Eduardo H. G.; Bandeira, Valdir; Jardim, Márcio L.; Abath, Frederico G. C. // Journal of Infectious Diseases;3/15/2004, Vol. 189 Issue 6, p1018 

    Background. It is uncertain whether Leishmania parasites ever disappear after clinical cure of American cutaneous leishmaniasis (ACL). Recently, sensitive molecular techniques have allowed the identification of Leishmania parasites directly in specimens from patients' scars. Methods. Scars of 32...

  • Molecular identification of Theileria parasites of northwestern Chinese Cervidae. Youquan Li; Ze Chen; Zhijie Liu; Junlong Liu; Jifei Yang; Qian Li; Yaqiong Li; Shuangqing Cen; Guiquan Guan; Qiaoyun Ren; Jianxun Luo; Hong Yin // Parasites & Vectors;2014, Vol. 7 Issue 1, p1 

    Background Theileria and Babesia protozoan parasites are transmitted mainly by tick vectors. These parasites cause heavy economic losses to the live-stock industry, as well as affecting the health of wild animals in parasite-endemic areas. Identification of infectious agents in wild animals is...

  • Detection of DNA from Leishmania (Viannia): Accuracy of Polymerase Chain Reaction for the Diagnosis of Cutaneous Leishmaniasis. Neitzke-Abreu, Herintha Coeto; Venazzi, Mateus Sabaini; Bernal, Marcos Vinicius Zandonadi; Reinhold-Castro, Kárin Rosi; Vagetti, Fernanda; Mota, Camila Alves; Silva, Naielly Rodrigues; Aristides, Sandra Mara Alessi; Silveira, Thaís Gomes Verzignassi; Lonardoni, Maria Valdrinez Campana // PLoS ONE;Jul2013, Vol. 8 Issue 7, p1 

    Cutaneous leishmaniasis (CL) can occur in skin and mucosa, causing disfiguring lesions. The laboratory diagnosis of CL involves immunological methods and optical detection of the parasite, al of which have limitations. There is a need for more effective diagnostic methods for CL which wil allow...

  • The emergence of Leishmania major and Leishmania donovani in southern Turkey. Koltas, Ismail S.; Eroglu, Fadime; Alabaz, Derya; Uzun, Soner // Transactions of the Royal Society of Tropical Medicine & Hygiene;Mar2014, Vol. 108 Issue 3, p154 

    Background In southern Turkey, Leishmania tropica and L. infantum are both the causative agents of cutaneous leishmaniasis (CL) and visceral leishmaniasis (VL), respectively. However, L. major and L. donovani were known to exist after the influx of Syrian refugees. Methods Between the years of...

  • Incidence Trend of Rural Cutaneous Leishmaniasis in Gonbad-e- Qabus City, (Golestan, Iran) during 2009-2012. Pagheh, Abdol Sattar; Fakhar, Mahdi; Mesgarian, Fatemeh; Rahimi-esboei, Bahman; Badiee, Farhad // Journal of Mazandaran University of Medical Sciences (JMUMS);Sep2013, Vol. 23 Issue 104, p26 

    Background and purpose: Recently, incidence of cutaneous leishmaniasis (CL) has been increased. The present study aimed to investigate the incidence trend of CL in Gonbad-e-Qabus, (Golestan, Iran). Materials and methods: This descriptive study was carried out for patients referring to health...

  • A Prospective Cohort Study of Cutaneous Leishmaniasis Risk and Opium Addiction in South Eastern Iran. Aflatoonian, Mohammad Reza; Sharifi, Iraj; Hakimi Parizi, Maryam; Fekri, Ali Reza; Aflatoonian, Behnaz; Sharifi, Maryam; Khosravi, Ahmad; Khamesipour, Ali; Sharifi, Hamid // PLoS ONE;Feb2014, Vol. 9 Issue 2, p1 

    Opium addiction and cutaneous leishmaniasis (CL) are endemic in different parts of Iran, particularly in Bam, where a massive earthquake occurred. This study was designed to compare the incidence rate and severity of CL cases among opium addicted and non-addicted individuals in south-eastern...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics