TITLE

The gammaPE Complex Contains Both SATB1 and HOXB2 and Has Positive and Negative Roles in Human gamma-Globin Gene Regulation

AUTHOR(S)
Case, Scott S.; Huber, Philippe; Lloyd, Joyce A.
PUB. DATE
November 1999
SOURCE
DNA & Cell Biology;Nov99, Vol. 18 Issue 11, p805
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A large nuclear protein complex, termed gammaPE (for gamma-globin promoter and enhancer binding factor), binds to five sites located 5' and 3' of the human gamma-globin gene. Two proteins, SATB1 (special A-T-rich binding protein 1) and HOXB2, can bind to gammaPE binding sites. SATB1 binds to nuclear matrix-attachment sites, and HOXB2 is a homeodomain protein important in neural development that is also expressed during erythropoiesis. The present work showed that antisera directed against either SATB1 or HOXB2 reacted specifically with the entire gammaPE complex in electrophoretic mobility shift assays (EMSAs), suggesting that the two proteins can bind to the gammaPE binding site simultaneously. When SATB1 or HOXB2 was expressed in vitro, they could bind independently to gammaPE binding sites in EMSA. Interestingly, the proteins expressed in vitro competed effectively with each other for the gammaPE binding site, suggesting that this may occur under certain conditions in vivo. Transient cotransfections of a HOXB2 cDNA and a gamma-globin-luciferase reporter gene construct into cells expressing SATB1 suggested that SATB1 has a positive and HOXB2 a negative regulatory effect on transcription. Taking into account their potentially opposing effects and binding activities, SATB1 and HOXB2 may modulate the amount of gamma-globin mRNA expressed during development and differentiation.
ACCESSION #
6778155

 

Related Articles

  • Matrin 3 and HIV Rev Regulation of mRNA.  // Retrovirology;2011, Vol. 8 Issue 1, p62 

    The article presents the author's views on two papers the nuclear matrix protein, MATR3, that was published in previous issue of the journal. The pappers discussed MATR3, as a co-factor used by HIV-1 Rev protein for facilitating nuclear export as well as translation of both unspliced and...

  • Characterization of the nuclear matrix targeting sequence (NMTS) of the BPV1 E8/E2 protein – the shortest known NMTS. Sankovski, Eve; Karro, Kristiina; Sepp, Mari; Kurg, Reet; Ustav, Mart; Abroi, Aare // Nucleus (1949-1034);2015, Vol. 6 Issue 4, p289 

    Technological advantages in sequencing and proteomics have revealed the remarkable diversity of alternative protein isoforms. Typically, the localization and functions of these isoforms are unknown and cannot be predicted. Also the localization signals leading to particular subnuclear...

  • Stability of Nucleolin Protein as the Basis for the Differential Expression of Nucleolin mRNA and Protein during Serum Starvation. Kim, Steven K.; Srivastava, Meera // DNA & Cell Biology;Mar2003, Vol. 22 Issue 3, p171 

    Nucleolin is a nucleolar phosphoprotein that plays a direct role in ribosome biogenesis. Our aim was to determine how its activity as a growth-promoting factor is coordinated with, if not regulated by, the cell cycle machinery. In serum starting and then rescuing these cells with serum, we found...

  • A new view of mRNA export: Separating the wheat from the chaff. Reed, Robin; Magni, Keith // Nature Cell Biology;Sep2001, Vol. 3 Issue 9, pE201 

    Focuses on the two major classes of proteins playing key roles in the messenger RNA (mRNA) export. Features of the nuclear RNA-binding (hnRNP) proteins and the non-hnRNP mRNA-associated proteins; Conserved mRNA export factors; Other splicing-dependent mRNA-associated proteins; Model for mRNA...

  • bicistronic.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p259 

    The definition of the medical term "bicistronic," which refers to the capability of making two proteins from a single messenger RNA molecule, is presented.

  • Widespread predicted nonsense-mediated mRNA decay of alternatively-spliced transcripts of human normal and disease genes. R.E. Green; B.P. Lewis; R.T. Hillman; M. Blanchette; L.F. Lareau; A.T. Garnett; D.C. Rio; S.E. Brenner // Bioinformatics;Jan2009 Supplement, Vol. 19, p118 

    We have recently shown that a third of reliably-inferred alternative mRNA isoforms are candidates for nonsense-mediated mRNA decay (NMD), an mRNA surveillance system (Lewis et al., 2003; Proc. Natl Acad. Sci. USA, 100, 189-192). Rather than being translated to yield protein, these transcripts...

  • Gene structure-based splice variant deconvolution using a microarry platform. D.C. Kulp; H. Wang; D. Haussler; E. Hubbell; J-s. Hu; G. Mei; M. Cline; G. Lu; T. Clark; M.A. Siani-Rose; M. Ares // Bioinformatics;Jan2009 Supplement, Vol. 19, p315 

    Motivation: Alternative splicing allows a single gene to generate multiple mRNAs, which can be translated into functionally and structurally diverse proteins. One gene can have multiple variants coexisting at different concentrations. Estimating the relative abundance of each variant is...

  • The transorientation hypothesis for codon recognition during protein synthesis. Simonson, Anne B.; Lake, James A. // Nature;3/21/2002, Vol. 416 Issue 6878, p281 

    Examines the transorientation hypothesis for codon of messenger RNA during protein synthesis. Rotation of transfer RNA binding site; Formation of ribosomal protein L11; Interaction between initiator and methionine elongator anti-codon loop structures.

  • Errors in genomics and proteomics. Jansen, Ritsert C.; Nap, Jan-Peter; Mlynárová, Ludmila // Nature Biotechnology;Jan2002, Vol. 20 Issue 1, p19 

    Postulates the existence of 'error pipelines' that mask a biologically relevant correlation between mRNA and protein levels. Assays of mRNA; Lack of correlation between RNA and protein data.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics