TITLE

Infection with Vaccinia Virus Alters Regulation of Cell Cycle Progression

AUTHOR(S)
Wali, Anil; Strayer, David S.
PUB. DATE
November 1999
SOURCE
DNA & Cell Biology;Nov99, Vol. 18 Issue 11, p837
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The effect of vaccinia virus (VV) on cell cycle progression and its regulators was studied. Infected cultures showed significantly increased transit through G[sub 1], decreasing the percentage of cells in G[sub 1] and increasing the percentage in S phase. The numbers of cells in G[sub 2]/M were not affected. Because of the increased S-phase fraction at the expense of G[sub 1], expression of cyclins and cyclin-dependent kinases (Cdks) that regulate cell cycle checkpoints was examined. Transcripts for cyclins A and B, Cdk2, and Cdc2 were decreased in VV-infected cells as infection progressed. The amounts of p53 and p27 proteins decreased after 12 and 24 h of infection, respectively. The Cdc2 and Cdk2 protein levels were decreased with increasing time after infection. Taken together, these findings would be expected to lead to more cells in S phase and G[sub 2]/M, as was observed. Therefore, VV actively modulates expression of cellular regulators of the cell cycle and alters cell cycle progression.
ACCESSION #
6778152

 

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