TITLE

Bacterial identification in the diagnostic laboratory: How much is enough?

AUTHOR(S)
Kootallur, B. N.; Thangavelu, C. P.; Mani, M.
PUB. DATE
October 2011
SOURCE
Indian Journal of Medical Microbiology;Oct2011, Vol. 29 Issue 4, p336
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The major impetus for bacterial identification came after the advent of solid culture media. Morphological appearance of bacterial colonies was often sufficient for their identification in the laboratory. Even in modern times, preliminary identification of most cultivable bacteria is based on such morphological characters. Advances have been made media for the presumptive identification of common organisms encountered in clinical samples. Phenotypic characterisation of bacteria with, physiological tests with a battery of biochemical tests differentiate related bacterial genera as well as confirm their identity. Each laboratory can select its own method(s) of identification, provided they are based on scientific / epidemiological evidence; clinical laboratory and standards institute (CLSI) is a widely accepted organization and laboratories in many parts of the world follow its recommendations for bacterial identification. Some of the latest advances in identification include Matrix Assisted Laser Desorption Ionization -- Time of Flight Mass Spectroscopy (MALDI-TOF) is a state of art facility used for fast and reliable species-specific identification of bacteria including Mycobacteria and fungi including yeasts. However the single most important factor that decides the method of bacterial identification in any laboratory is the cost involved. In the final analysis, selection of tests for bacterial identification should be based on their standardization with proper scientific basis. Considering the cost and lack of easy availability of commercial kits, we have put forward a simplified and rapid method of identification for most commonly encountered bacterial pathogens causing human infection in India.
ACCESSION #
67763048

 

Related Articles

  • Cryptococcus.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p552 

    A definition of the medical term "Cryptococcus," which refers to a genus of pathogenic yeastlike fungi, is presented.

  • Paracoccidioides.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p1701 

    A definition of the medical term "Paracoccidiodes," which refers to a genus of yeast-like fungi, is presented.

  • FUNGAL AND YEAST INFECTIONS. Buxton, P.K. // British Medical Journal (Clinical Research Edition);2/6/1988, Vol. 296 Issue 6619, p420 

    Presents lists of different fungal and yeast infections. Description of fungal infections; Occurrence and types of infections in different age groups; Regions of the body that the infections normally occur.

  • Lipid Composition of the Arthrospores, Yeastlike Cells, and Mycelium of the Fungus Mucor hiemalis. Mysyakina, I. S.; Funtikova, N. S. // Microbiology (00262617);Jul2001, Vol. 70 Issue 4, p403 

    The fungus Mucor hiemalisF-1156, which is believed to be monomorphic, was found to be able to grow dimorphically in a liquid medium that is free of chemical agents influencing morphogenesis. The growing mycelium produced arthrospores in large amounts. The lipids of the mycelium, yeastlike...

  • Modulatory Effect of the Lectin-Binding Component, Added to Gel, on Adhesion of Yeast-Like Candida Fungi In Vivo. Anokhina, I.; Kravtsov, E.; Sachivkina, N.; Yashina, N.; Dalin, M.; Semkina, O. // Bulletin of Experimental Biology & Medicine;Nov2011, Vol. 152 Issue 1, p86 

    The lectin-binding component added to gel was studied in vivo as a potential therapeutic and preventive agent in experimental vaginal candidiasis. Laboratory studies demonstrated its antimycotic effect in relapsing vaginal candidiasis in mice.

  • Herbal and Nutritional Approaches to Candida. Dunbar, Jo // Positive Health;Nov2004, Issue 105, p30 

    Provides information about candida overgrowths. Description for candida; Symptoms associated with candida; Treatments.

  • STUDIES OF THE FLORA OF THE MOUTH I. YEAST-LIKE ORGANISMS: SOME MORPHOLOGICAL AND PHYSIOLOGICAL CHARACTERS. Lilienthal, B.; Goldsworthy, N. E. // Australian Journal of Experimental Biology & Medical Science;May1950, Vol. 28 Issue 3, p261 

    The morphological and physiological characteristics are described for 166 strains of yeast-like organisms isolated from the mouth. These strains were studied in three groups according to their origin, viz. two groups from white Australians and one group from New Guinea natives. The strains were...

  • Eremothecium coryli and E. ashbyi cause yeast spot of azuki bean. Kimura, Shigemitsu; Tokumaru, Susumu; Kuge, Kazuhiko // Journal of General Plant Pathology;Aug2009, Vol. 75 Issue 4, p322 

    Yeast-like fungi were isolated from lesions on azuki bean (cv. Shin-Kyotodainagon) seeds that had been sucked by bean bugs in Kyoto Prefecture, Japan. On the basis of morphological and physiological characteristics and sequence data of the internal transcribed spacer (ITS) regions including the...

  • The Yeast Fungus Trichosporon lactis Found as an Epizoic Colonizer of Dung Beetle Exoskeletons. Górz, Andrzej; Boroń, Piotr // Microbial Ecology;Feb2016, Vol. 71 Issue 2, p422 

    The study on the biology and biodiversity of coprophagous Scarabaeoidea carried out in the Polish Carpathians revealed the occurrence of unusual epizoic excrescences on various dung beetles species of the genus Onthophagus. The excrescences occur on the elytra, prothorax, and head of the studied...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics