TITLE

In silico feedback for in vivo regulation of a gene expression circuit

AUTHOR(S)
Milias-Argeitis, Andreas; Summers, Sean; Stewart-Ornstein, Jacob; Zuleta, Ignacio; Pincus, David; El-Samad, Hana; Khammash, Mustafa; Lygeros, John
PUB. DATE
December 2011
SOURCE
Nature Biotechnology;Dec2011, Vol. 29 Issue 12, p1114
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We show that difficulties in regulating cellular behavior with synthetic biological circuits may be circumvented using in silico feedback control. By tracking a circuit's output in Saccharomyces cerevisiae in real time, we precisely control its behavior using an in silico feedback algorithm to compute regulatory inputs implemented through a genetically encoded light-responsive module. Moving control functions outside the cell should enable more sophisticated manipulation of cellular processes whenever real-time measurements of cellular variables are possible.
ACCESSION #
67759367

 

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