TITLE

Artificial Self-Sufficient P450 in Reversed Micelles

AUTHOR(S)
Hirakawa, Hidehiko; Kamiya, Noriho; Kawarabayasi, Yutaka; Nagamune, Teruyuki
PUB. DATE
May 2010
SOURCE
Molecules;May2010, Vol. 15 Issue 5, p2935
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Cytochrome P450s are heme-containing monooxygenases that require electron transfer proteins for their catalytic activities. They prefer hydrophobic compounds as substrates and it is, therefore, desirable to perform their reactions in non-aqueous media. Reversed micelles can stably encapsulate proteins in nano-scaled water pools in organic solvents. However, in the reversed micellar system, when multiple proteins are involved in a reaction they can be separated into different micelles and it is then difficult to transfer electrons between proteins. We show here that an artificial self-sufficient cytochrome P450, which is an enzymatically crosslinked fusion protein composed of P450 and electron transfer proteins, showed micelle-size dependent catalytic activity in a reversed micellar system. Furthermore, the presence of thermostable alcohol dehydrogenase promoted the P450-catalyzed reaction due to cofactor regeneration.
ACCESSION #
67705548

 

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