Generation of recombinant single-chain antibodies neutralizing the cytolytic activity of vaginolysin, the main virulence factor of Gardnerella vaginalis

January 2011
BMC Biotechnology;2011, Vol. 11 Issue 1, p100
Academic Journal
The article presents a research in which researchers constructed recombinant single-chain variable fragments of immunoglobulins (scFvs) by using hybridomas producing well characterized monoclonal antibodies (MAbs) 9B4 and 23A2 with the most potent vaginolysin (VLY) neutralizing activity. It is reported that, the researchers used indirect enzyme-linked immunosorbent assay (ELISA) technique to verify antigen-binding activity of purified scFvs.


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