TITLE

The influence of hyaluronic acid on vascular endothelial cell proliferation and the relationship with ezrin/merlin expression

AUTHOR(S)
Mo, Wei; Yang, Cuixia; Liu, Yiwen; He, Yiqing; Wang, Yingzhi; Gao, Feng
PUB. DATE
December 2011
SOURCE
Acta Biochimica et Biophysica Sinica;Dec2011, Vol. 43 Issue 12, p930
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
It has been established that hyaluronic acid (HA) glycans (nHA) and oligosaccharide (oHA) exert different effects on the biological function of the vascular endothelial cell (EC), resulting in altered regulation of angiogenesis. However, the specific mechanism is still unclear. Our study focused on the effects of nHA and oHA on the ezrin and merlin proteins in EC. The expression of ezrin and merlin was silenced by siRNA, and the regulation on EC growth as well as the mRNA expression and activation (phosphorylation) of ezrin and merlin stimulated by oHA and nHA was investigated. The results revealed that when treated with nHA, there was no significant change in ezrin expression or activation. After being treated with oHA, the expression and activation of ezrin were definitively increased whereas there were no obvious changes in merlin expression (including its phosphorylation). With ezrin expression silenced, the expression of merlin as well as its phosphorylation levels in nHA-stimulated human umbilical vein endothelial cells were notably elevated, while there was no significant change induced by oHA. With merlin expression silenced, no obvious change was found in the expression of ezrin (including its phosphorylation) induced by nHA. Conversely, the expression of ezrin and its activation was significantly improved after being treated with oHA. The results suggest that the mechanism for the promotion of EC proliferation by oHA is likely related to the expression and activation of ezrin, and the inhibition of EC proliferation by nHA is likely related to the expression and activation of merlin.
ACCESSION #
67627290

 

Related Articles

  • Expression of cell adhesion proteins and proteins related to angiogenesis and fatty acid metabolism in benign, atypical, and anaplastic meningiomas. Alexandros Panagopoulos; Carmen Lancellotti; José Veiga; Paulo de Aguiar; Alison Colquhoun // Journal of Neuro-Oncology;Aug2008, Vol. 89 Issue 1, p73 

    Abstract  Most meningiomas are benign tumours of arachnoidal origin, although a small number have high proliferative rates and invasive properties which complicate complete surgical resection and are associated with increased recurrence rates. Few prognostic indicators exist for...

  • Expression and Control of Vascular Endothelial Cells: Proliferation and Differentiation by Fibroblast Growth Factors. Gospodarowicz, Denis // Journal of Investigative Dermatology;Aug89 Supplement, Vol. 93, p39S 

    Evidence from in vitro studies supports the concept that growth factors could be involved in the development and function of the vascular tree. Among the growth factors known to influence vascular endothelial cells' proliferation and differentiation, in vitro, are the fibroblast growth factors....

  • Expression of bcl-2 and survivin gene products in different stages of vessel differentiation in hemangiomas. Tan Hai-song; Qin Xing-jun; Wang Xu-kai // Shanghai Journal of Stomatology;Apr2008, Vol. 17 Issue 2, p204 

    PURPOSE: To investigate the relationship between cell apoptosis and regression process of hemangiomas. METHODS: Forty four hemangiomas and 19 vascular malformations, confirmed by pathology without any previous treatments were involved. Hemangiomas were classified into two groups by histological...

  • Peroxisome Proliferator-Activated Receptor ?? (PPAR??) and Atherosclerosis. Gouni-Berthold, I.; Krone, W. // Current Drug Targets - Cardiovascular & Haematological Disorders;Dec2005, Vol. 5 Issue 6, p513 

    The peroxisome proliferator-activated receptors (PPARs) α, β/δ and γ are ligand-activated transcription factors belonging to the nuclear receptor superfamily. PPARs heterodimerize with the retinoid X receptor (RXR) and modulate the function of many target genes. They were originally...

  • Modulation by leptin or proliferation and apoptosis in vascular endothelial cells. Artwohl, M.; Roden, M.; Holzenbein, T.; Freudenthaler, A.; Waldhausl, W.; Baumgartner-Parzer, S.M. // International Journal of Obesity & Related Metabolic Disorders;Apr2002, Vol. 26 Issue 4, p577 

    Examines plasma leptin concentrations in proliferating and apoptotic vascular endothelial cells. Increased expression of apoptosis inhibitor bcl-2; Differential regulation of cell cycle checkpoint genes and the retinoblastoma protein.

  • Effects of cytokines on vascular endothelium: Their role in vascular and immune injury. Cotran, Ramzi S.; Pober, Jordan S. // Kidney International;Apr1989, Vol. 35 Issue 4, p969 

    Examines the effects of cytokines on vascular endothelium and their role in vascular and immune injury. Decrease in tissue plasminogen activator; Stimulation of surface expression of intracellular adhesion molecule-1; Involvement of endothelial cells in inflammatory reactions; Control of the...

  • A Polysaccharide Isolated from the Medicinal Herb Bletilla striata Induces Endothelial Cells Proliferation and Vascular Endothelial Growth Factor Expression in vitro. Chunming Wang; Jiantao Sun; Yi Luo; Weihua Xue; Huajia Diao; Lei Dong; Jiangning Chen; Junfeng Zhang // Biotechnology Letters;Apr2006, Vol. 28 Issue 8, p539 

    A polysaccharide from the traditional Chinese medicinal herb, Bletilla striata (Thunb.) Reichb. f., was isolated, purified and characterized. It induced the proliferation of human umbilical vascular endothelial cells and the expression of vascular endothelial growth factor up to 156% and 147% of...

  • Reduced pathological angiogenesis and tumor growth in mice lacking GPR4, a proton sensing receptor. Wyder, Lorenza; Suply, Thomas; Ricoux, Bérangère; Billy, Eric; Schnell, Christian; Baumgarten, Birgit; Maira, Sauveur; Koelbing, Claudia; Ferretti, Mireille; Kinzel, Bernd; Müller, Matthias; Seuwen, Klaus; Ludwig, Marie-Gabrielle // Angiogenesis;Dec2011, Vol. 14 Issue 4, p533 

    The G protein-coupled receptor GPR4 is activated by acidic pH and recent evidence indicates that it is expressed in endothelial cells. In agreement with these reports, we observe a high correlation of GPR4 mRNA expression with endothelial marker genes, and we confirm expression and acidic pH...

  • Involvement of Plasmalogens in Post-Natal Retinal Vascular Development. Saab, Sarah; Buteau, Bénédicte; Leclère, Laurent; Bron, Alain M.; Creuzot-Garcher, Catherine P.; Bretillon, Lionel; Acar, Niyazi // PLoS ONE;Jun2014, Vol. 9 Issue 6, p1 

    Objective: Proper development of retinal blood vessels is essential to ensure sufficient oxygen and nutrient supplies to the retina. It was shown that polyunsaturated fatty acids (PUFAs) could modulate factors involved in tissue vascularization. A congenital deficiency in ether-phospholipids,...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics