TITLE

Effect of Copper on the Expression of TGF-β in Incubated Chondrocytes of Newborn Pigs

AUTHOR(S)
Zhu, Xiaoyan; Wang, Jianguo; Xie, Guanghong; Feng, Haihua; Li, Xiaobing; Liu, Lei; Wang, Xiaoxu; Li, Dongna; Liu, Zhaoxi; Qian, Jian; Wang, Zhe; Liu, Guowen
PUB. DATE
December 2011
SOURCE
Biological Trace Element Research;Dec2011, Vol. 143 Issue 3, p1461
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
This experiment was conducted to measure the effect of copper supplementation on TGF-β gene expression in chondrocytes of newborn pigs. Chondrocytes were cultured in media containing 15% fetal calf serum supplemented with 0, 15.6, 31.2, and 62.5 μmol/L copper in 90-mm culture plate. Total RNA was isolated from chondrocytes, and TGF-β cDNA was synthesized, amplified, and sequenced. The expression level of TGF-β was examined by reverse transcription polymerase chain reaction. The results showed that the sequence of the cloned TGF-β gene was 99.4% identical to that in GenBank. The expression of TGF-β increased in culture media added with final concentration of 15.6, 31.2, and 62.5 μmol/L copper. In this study, the optimal copper concentration and optimal culture time for the highest level of TGF-β expression were 31.2 μmol/L and 48 h, respectively.
ACCESSION #
67339791

 

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