Detection of Aeromonas hydrophila in water using PCR

November 2011
Journal: American Water Works Association;Nov2011, Vol. 103 Issue 11, p59
Academic Journal
Aeromonas hydrophila is an emerging pathogen being detected in drinking water. Classical pathogen identification methods take five to seven days—including inoculating into a medium, culturing, staining, and confirming pathogen existence via biochemical or serological tests. Although accepted for decades, these methods are extremely labor-intensive and time-consuming. Polymerase chain reaction (PCR), however, provides rapid (6 h) and inexpensive detection of pathogens and could significantly reduce the time required to identify A. hydrophila strains. The prevalence and distribution of Aeromonas in aquatic environments, its role as a contaminant for drinking water supplies, and its potential for pathogenicity are public health concerns. A. hydrophila causes human diseases associated with a variety of health issues, including septicemia, wound infections, meningitis, peritonitis, and hepatobiliary infections. A PCR-based method could provide a powerful complement to conventional methods for more accurate risk assessment and monitoring of pathogenic bacteria in drinking water. The ability to rapidly detect A. hydrophila would be extremely useful not only for routine assessment of water quality, but also for assessments of water quality during the water treatment processes.


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