TITLE

Characterization of novel glycolipid antigens with an α-galactose epitope in lactobacilli detected with rabbit anti-Lactobacillus antisera and occurrence of antibodies against them in human sera

AUTHOR(S)
Iwamori, Masao; Sakai, Akihiro; Minamimoto, Norihito; Iwamori, Yuriko; Tanaka, Kyoko; Aoki, Daisuke; Adachi, Shigeki; Nomura, Taisei
PUB. DATE
November 2011
SOURCE
Journal of Biochemistry;Nov2011, Vol. 150 Issue 5, p515
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Anti-Lactobacillus johnsonii (LJ) antisera generated by immunization of rabbits with LJ reacted with glyceroglycolipids in LJ, i.e. dihexaosyl diacylglycerol (DH-DG), trihexaosyl DG (TH-DG) and tetrahexaosyl DG (TetH-DG), whose reactivities with antisera increased proportionally with longer carbohydrate chains of glycolipids. Structural analyses of glycolipids from LJ revealed that DH-DG was Galα1-2Glcα1-3′DG, and TH-DG and TetH-DG were novel derivatives of it with α-Gal at the non-reducing terminal, i.e. Galα1-6Galα1-2Glcα1-3′DG and Galα1-6Galα1-6Galα1-2Glcα1-3′DG, respectively. DH-DG was commonly present in several lactobacilli examined, but TetH-DG was restricted to LJ, L. intestinalis and L. reuteri, while the TH-DGs from L. casei were Glc1-6Galα1-2Glcα1-3′DG and an esterified derivative of it, Glc1-6Galα1-2Glc(6-fatty acid)α1-3′DG, as reported in the literature. Anti-LJ antisera reacted with TH-DG and esterified TH-DG from L. casei to lesser extents, but not at all with gentibiosyl DG from Staphylococcus epidermidis or kojibiosyl DG from Streptococcus salivalis or sphingoglycolipids containing α-Gal residues. The major molecular species of glycolipids obtained from lactobacilli were 11-octadecenoic and 11,12-methylene-octadecanoic acids-containing ones. Also, human IgM antibodies against TH-DG and TetH-DG from LJ were detected in human sera, with various antibody titres, indicating that an immune reaction to symbiotic lactobacilli occurs against their glycolipid antigens, TH-DG and TetH-DG.
ACCESSION #
67007526

 

Related Articles

  • Ramoplanin: A 16686, A 16686A, MDL 62198.  // Drugs in R&D;2002, Vol. 3 Issue 4, p271 

    Adis Comments Ramoplanin [A 16686, A 16686A, MDL 62198], originated by Biosearch Italia, is a glycolipopeptide antibacterial agent derived from Actinoplanes spp. with bactericidal activity primarily against Gram-positive organisms. Ramoplanin kills microbes by blocking bacterial cell wall...

  • Conversion of rice straw to bio-based chemicals: an integrated process using Lactobacillus brevis. Jae-Han Kim; Block, David; Shoemaker, Sharon; Mills, David // Applied Microbiology & Biotechnology;May2010, Vol. 86 Issue 5, p1375 

    Commercialization of lignocellulosic biomass as a feedstock for bio-based chemical production is problematic due to the high processing costs of pretreatment and saccharifying enzymes combined with low product yields. Such low product yield can be attributed, in large part, to the incomplete...

  • Lactobacillus.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p1288 

    A definition of the term "Lactobacillus," which refers to a genus of gram-positive, anaerobic bacilli of the Lactobacillaceae family, is presented.

  • Interactions of Lactobacilli with Pathogenic Streptococcus pyogenes. Westbroek, Mark L.; Davis, Crystal L.; Fawson, Lena S.; Price, Travis M. // Infectious Diseases in Obstetrics & Gynecology;2010, Vol. 2010, Special section p1 

    Objective. To determine whether (1) a decreased concentration of Lactobacilli allows S. pyogenes to grow; (2) S. pyogenes is able to grow in the presence of healthy Lactobacillus concentrations; (3) S. pyogenes is capable of inhibiting Lactobacilli. Methods. One hundred fifty patient samples of...

  • The glycolytic genes pfk and pyk from Lactobacillus casei are induced by sugars transported by the phosphoenolpyruvate:sugar phosphotransferase system and repressed by CcpA. Viana, Rosa; Pérez-Martínez, Gaspar; Deutscher, Josef; Monedero, Vicente // Archives of Microbiology;Jun2005, Vol. 183 Issue 6, p1 

    In Lactobacillus casei BL23, phosphofructokinase activity was higher in cells utilizing sugars transported by the phosphoenolpyruvate:sugar phosphotransferase system (PTS). The phosphofructokinase gene ( pfk) was cloned from L. casei and shown to be clustered with the gene encoding pyruvate...

  • Probiotics or prebiotics? Turner, Natasha // Alive: Canada's Natural Health & Wellness Magazine;Nov2005, Issue 277, p50 

    The article focuses on the health benefits derived from probiotics. Beneficial bacteria found in probiotics can keep the digestive system functioning normally. Research has found intestinal microflora are cancer-protective, immune-enhancing, and anti-inflammatory. Enriching microflora with...

  • Fermentation of a milk-soymilk andLycium chinenseMiller mixture using a new isolate ofLactobacillus paracaseisubsp.paracaseiNTU101 andBifidobacterium longum. Lin, Fu-Mei; Chiu, Chiu-Hsia; Pan, Tzu-Ming // Journal of Industrial Microbiology & Biotechnology;Dec2004, Vol. 31 Issue 12, p559 

    A milk-soymilk mixture was fermented usingLactobacillus paracaseisubsp.paracaseiNTU101 andBifidobacterium longumBCRC11847 at different inoculum ratios (1:1, 1:2, 1:5, 2:1, and 5:1). When the inoculum ratio was 1:2, the cell numbers of both strains were balanced after 12 h of cultivation. The pH...

  • Evaluation of nitric oxide production by lactobacilli. Xu, J.; Verstraete, W. // Applied Microbiology & Biotechnology;Aug2001, Vol. 56 Issue 3/4, p504 

    Six strains of Lactobacillus fermentum and Lactobacillus plantarum were investigated for nitric oxide (NO) production. First, the potential presence of NO synthase was examined. None of the strains of L. fermentum and L. plantarum examined produced NO from L-arginine under aerobic conditions....

  • Effect of Lactobacillus sakei Lb706 on Behavior of Listeria monocytogenes in Vacuum-Packed Rainbow Trout Fillets. Hİsar, şükriye Aras; Kaban, Güzin; Hısar, Olcay; Yanik, Telat; Kaya, Mükerrem // Turkish Journal of Veterinary & Animal Sciences;2005, Vol. 29 Issue 4, p1039 

    The effect of a bacteriocin-producing strain, Lactobacillus sakei Lb706, on the behavior of Listeria monocytogenes in rainbow trout fillets stored under vacuum at 4 ºC for 14 days and at 10 ºC for 5 days was investigated. At 4 ºC, growth of L. monocytogenes was significantly inhibited...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics