Calibration method of tilt and azimuth angles for alignment of TEM tomographic tilt series

Hayashida, Misa; Terauchi, Shinya; Fujimoto, Toshiyuki
October 2011
Review of Scientific Instruments;Oct2011, Vol. 82 Issue 10, p103706
Academic Journal
This paper describes the calibration method of the tilt and azimuth angles of specimen using a digital protractor and a laser autocollimator for alignment of electron tomography. It also suggests an easy method to check whether the specimen is tilted by 180.0°, and whether the azimuth angle is 0.0°; the method involves the use of two images of a rod-shaped specimen collected before and after a 180.0° tilt. The method is based on the assumption that these images are symmetric about the tilt axis when the azimuth angle is 0.0°. In addition, we used an experiment to demonstrate the effect of the incorrect angles on reconstructed images and simulated the image quality against distance away from tilt axis.


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