Variation of genetic diversity over time in local Italian chicken breeds undergoing in situ conservation

Zanetti, E.; De Marchi, M.; Abbadi, M.; Cassandro, M.
October 2011
Poultry Science;Oct2011, Vol. 90 Issue 10, p2195
Academic Journal
The aim of this study was to analyze the variation of different genetic diversity measures in 5 Italian local chicken breeds over a 4-yr period of conservation. The local breeds were Ermellinata di Rovigo, Padovana, Pépoi, Robusta Lionata, and Robusta Maculata. A total of 368 samples were chosen for the analysis among the birds hatched in the years 2002 and 2006. Genetic variation over 30 microsatellite loci was analyzed. All 30 microsatellites were polymorphic, with a total number of alleles equal to 251, a mean (±SD) of 8.367 ± 3.378 across populations and 3.233 ± 1.338 within population. After 4 yr of conservation, a loss of alleles occurred for all the microsatellites, with the exception of 4 loci. The total number of alleles and expected heterozygosity estimates significantly decreased during the 4 yr of conservation, whereas no significant differences were detected for the microsatellites polymorphism information content or for the observed heterozygosity estimates. A decrease of the inbreeding coefficient occurred for all the breeds, with the exception of Padovana and Robusta Lionata. All populations showed evidence of a persistent significant deviation from Hardy-Weinberg equilibrium caused by an excess of homozygotes, except Robusta Maculata, which reached the equilibrium in 2006. For Pépoi, Padovana, Ermellinata di Rovigo, and Robusta Maculata, molecular coancestry increased significantly (P < 0.001) after the 4 yr of conservation. No evident genetic structures were detectable within breed, both for 2002 and for 2006 individuals. However, a slight increase in the proportion of membership for each breed had occurred in the year 2006 compared with the data obtained in the year 2002. As consequence, within breed, individuals in 2006 appear more homogeneous, producing clearer, more distinctive and separated groups. Molecular markers analysis helped us monitor the genetic variability of local breeds involved in a conservation scheme, enabling the planning of new strategies for the improvement of in situ conservation schemes.


Related Articles

  • A microsatellites-based survey on the genetic structure of two Italian local chicken breeds. Bianchi, Matteo; Ceccobelli, Simone; Landi, Vincenzo; Di Lorenzo, Piera; Lasagna, Emiliano; Ciocchetti, Martina; Şahin, Emine; Mugnai, Cecilia; Panella, Francesco; Sarti, Francesca Maria // Italian Journal of Animal Science;Jul2011, Vol. 10 Issue 3, p205 

    The biodiversity safeguard is an important goal of poultry production in every developed country. Nowadays, the high chicken meat demand from the world market has been leading to a large spread of strongly producing commercial chicken lines. The creation of these standard types is causing a...

  • Genetic variation of indigenous chicken breeds in China and a Recessive White breed using AFLP fingerprinting. Yushi Gao; Yunjie Tu; Haibin Tong; Kehua Wang; Xiujun Tang; Kuanwei Chen // South African Journal of Animal Science;2008, Vol. 38 Issue 3, p193 

    Amplified fragment length polymorphism (AFLP) using six marker combinations were applied to detect genetic variation and phylo genetic relationships among 12 indigenous Chinese chicken breeds and a Recessive White chicken breed introduced from France. The DNA was pooled for each group....

  • Time-Dependent Changes in the Genetic Diversity and Structure of the Onagadori Breed of Chickens Based on Microsatellite DNA Polymorphisms. Takao Oka; Ryo Tadano; Tatsuhiko Goto; Jun-ichi Shiraishi; Osman, Sayed A. M.; Naohiro Nagasaka; Takashi Bungo; Teruo Maeda; Masahide Nishibori; Tetsuya Umino; Masaoki Tsudzuki // Journal of Poultry Science;Jul2014, Vol. 51 Issue 3, p262 

    To conserve the Tosa-no-Onagadori (briefly Onagadori), one of the native Japanese chicken breeds designated as a Special National Natural Treasure of Japan, time-dependent changes in genetic diversity and structure were analyzed based on 20 microsatellites. From three varieties (black-breasted...

  • Isolation and characterization of the first microsatellite markers for the endangered swan mussel Anodonta cygnea L. (Bivalvia: Unionoidea). Geist, Juergen; Geismar, Jutta; Kuehn, Ralph // Conservation Genetics;Jun2010, Vol. 11 Issue 3, p1103 

    Freshwater molluscs are globally critically threatened, yet little is known about their genetic diversity. We describe the isolation and characterization of the first microsatellite markers for the European swan mussel, Anodonta cygnea. Nine loci were polymorphic in a panel of 110 individuals...

  • LEI0258 Microsatellite Variability in Khorasan, Marandi, and Arian Chickens. Nikbakht, Gholamreza; Esmailnejad, Atefeh; Barjesteh, Neda // Biochemical Genetics;Jun2013, Vol. 51 Issue 5/6, p341 

    Microsatellite LEI0258 is a genetic marker for chicken MHC haplotypes and can be used as an indicator of the influence of population genetics on immune responses. LEI0258 microsatellite variability in three Iranian indigenous chicken populations (Khorasan, Marandi, and Arian) was investigated....

  • Assessment of Genetic Relatedness of Crossbred Chicken Populations Using Microsatellite Markers. Chatterjee, Rudra Nath; Bhattacharya, Tarun K.; Dange, Meenakshi; Rajkumar, U. // Biochemical Genetics;Oct2010, Vol. 48 Issue 9/10, p727 

    To measure genetic relatedness between populations, for breeding purposes, we analyzed 170 birds from six crossbred populations of three pure lines of White Leghorn chickens, using 14 microsatellite markers. All the microsatellites were polymorphic, with 2–6 alleles. The mean number of...

  • Assignment of Fourteen Microsatellite to the Chicken Linkage Map. Jacobson, L.; Park, H.-B.; P. Wahlberg; Jiang, S.; Siegel, P. B.; Andersson, L. // Poultry Science;Nov2004, Vol. 83 Issue 11, p1825 

    A large mapping population, with 874 F2 individuals, was generated by reciprocally intercrossing 2 chicken lines. A genetic map of 2,426.6 cM comprising 25 linkage groups was established based on 145 microsatellite markers. Chromosome locations were assigned for 14 previously unmapped markers....

  • Analysis of genetic divergence between closely related lines of chickens. Tadano, R.; Nakamura, A.; Kino, K. // Poultry Science;Feb2012, Vol. 91 Issue 2, p327 

    The aim of the present study was to characterize genetic diversity within and differentiation between 5 closely related lines of Japanese-native chickens, the Nagoya breed, based on microsatellite polymorphisms. For 5 Nagoya lines, the mean number of alleles per locus, the observed...

  • The Mode of Evolution of Molecular Markers in Populations of House Mice Under Artificial Selection for Locomotor Behavior. Morgan, T. J.; Garland Jr., T.; Irwin, B. L.; Swallow, J. G.; Carter, P. A. // Journal of Heredity;May/Jun2003, Vol. 94 Issue 3, p236 

    Presents a study that examined the distribution of allelic variation at microsatellite and allozyme loci in hierarchical populations of house mice. Methodology; Determination of the heterozygosity of the house mice; Examination of the allozyme and microsatellite genotypes.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics