Dynamics of Reporter Gene Stimulation by HMG Box Proteins

Veilleux, Stéphane; Boissonneault, Guylain
March 2002
DNA & Cell Biology;Mar2002, Vol. 21 Issue 3, p199
Academic Journal
Overcoming local DNA rigidity is required to perform three-dimensional DNA–protein configuration at promoter regions. The abundant architectural nonhistone chromosomal HMG box proteins are nonsequence-specific; however, they have been established to specifically recognize distorted DNA. Using transient transfection to overexpress two different members of the HMGB-1/2 family of DNA architectural factors, we demonstrate that these proteins provide a general enhancement in reporter gene expression irrespective of the promoter being considered. Evidences are also provided indicating that stimulation may not be achieved by recruitment of the proteins by regulatory factors or as a consequence of major chromatin unfolding as previously suggested. Interestingly, the influence of the HMG box proteins under study was overridden when the promoters were either induced or stimulated by Trichostatin A (TSA) but recovered upon extended induction period. These results also support the concept that the architectural role of these proteins can contribute to the preinitiation complex assembly required for basal transcription, but to a much lesser extent to the poised promoter scaffolding characteristic of activated transcription.


Related Articles

  • Deacetylase inhibition promotes the generation and function of regulatory T cells. Ran Tao; de Zoeten, Edwin F.; Özkaynak, Engin; Chunxia Chen; Liqing Wang; Porrett, Paige M.; Bin Li; Turka, Laurence A.; Olson, Eric N.; Greene, Mark I.; Wells, Andrew D.; Hancock, Wayne W. // Nature Medicine;Nov2007, Vol. 13 Issue 11, p1299 

    Histone/protein deacetylases (HDACs) regulate chromatin remodeling and gene expression as well as the functions of more than 50 transcription factors and nonhistone proteins. We found that administration of an HDAC inhibitor (HDACi) in vivo increased Foxp3 gene expression, as well as the...

  • Chromosomal Proteins: Their Role in the Regulation of Gene Expression. Stein, Gary; Stein, Janet // BioScience;Aug1976, Vol. 26 Issue 8, p488 

    The article present evidence which indicates that chromosomal proteins are involved in the regulation of gene readout in eucaryotic cells. It informs that proteins associated with the eucaryotic genome are generally divided into two principal classeshistones and nonhistone chromosomal proteins....

  • HMGA2, the Architectural Transcription Factor High Mobility Group, Is Expressed in the Developing and Mature Mouse Cochlea. Smeti, Ibtihel; Watabe, Isabelle; Savary, Etienne; Fontbonne, Arnaud; Zine, Azel // PLoS ONE;Feb2014, Vol. 9 Issue 2, p1 

    Hmga2 protein belongs to the non-histone chromosomal high-mobility group (HMG) protein family. HMG proteins have been shown to function as architectural transcription regulators, facilitating enhanceosome formation on a variety of mammalian promoters. Hmga2 are expressed at high levels in...

  • Commentary on `Appliction of Exogenously Regulatable Promoter Systems to Transgenic Models for... Jazwinski, S. Michael // Journals of Gerontology Series A: Biological Sciences & Medical ;Jan99, Vol. 54A Issue 1, pB41 

    Comments on the study `Application of Exogenously Regulatable Promoter Systems to Transgenic Models for the Study of Aging,' by William W. Morgan and colleagues. Expression systems discussed in the study; Results of eliminating an activity or pathway; Details on the overexpression of a transgene.

  • Special content of this issue. Steinbüchel, Alexander // Applied Microbiology & Biotechnology;Mar2011, Vol. 89 Issue 5, p1265 

    An introduction to the journal is presented in which the editor discusses various reports published within the issue including one on the relation of promoters and vectors which are suited for Ralstonia eutropha gene expression by Budde and colleagues, one on polyhydroxyalkanoates (PHA) new...

  • IXR1 and HMO1 genes jointly control the level of spontaneous mutagenesis in yeast Saccharomyces cerevisiae. Fedorov, D. V.; Kovaltzova, S. V.; Peshekhonov, V. T; Korolev, V. G. // Russian Journal of Genetics;Jun2010, Vol. 46 Issue 6, p659 

    The yeast genes IXR1 and HMO1 encode proteins belonging to the family of chromatin nonhistone proteins, which are able to recognize and bind to irregular DNA structures. The full deletion of gene IXR1 leads to an increase in cell resistance to the lethal action of UV light, γ-rays, and MMS,...

  • A Y2H-seq approach defines the human protein methyltransferase interactome. Weimann, Mareike; Grossmann, Arndt; Woodsmith, Jonathan; Özkan, Ziya; Birth, Petra; Meierhofer, David; Benlasfer, Nouhad; Valovka, Taras; Timmermann, Bernd; Wanker, Erich E; Sauer, Sascha; Stelzl, Ulrich // Nature Methods;Apr2013, Vol. 10 Issue 4, p339 

    To accelerate high-density interactome mapping, we developed a yeast two-hybrid interaction screening approach involving short-read second-generation sequencing (Y2H-seq) with improved sensitivity and a quantitative scoring readout allowing rapid interaction validation. We applied Y2H-seq to...

  • Human UDP-Glucuronosyltransferase 2B7. Radominska-Pandya, A.; Little, J.M.; Czernik, P.J. // Current Drug Metabolism;Sep2001, Vol. 2 Issue 3, p283 

    UDP-Glucuronosyltransferases (UGTs) are glycoproteins, localized in endoplasmic reticulum (ER) and nuclear membranes, which catalyze the conjugation of a broad variety of lipophilic aglycon substrates with glucuronic acid using UDP-glucuronic acid (UDP-GlcUA) as the sugar donor. The major...

  • Increased level and duration of expression in muscle by co-expression of a transactivator using plasmid systems. Li, S; MacLaughlin, F C; Fewell, J G; Li, Y; Mehta, V; French, M F; Nordstrom, J L; Coleman, M; Belagali, N S; Schwartz, R J; Smith, L C // Gene Therapy;Dec99, Vol. 6 Issue 12, p2005 

    Skeletal muscle is an attractive target for gene therapies to treat either local or systemic disorders, as well as for genetic vaccination. An ideal expression system for skeletal muscle would be characterized by high level, extended duration of expression and muscle specificity. Viral...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics