Prognostic Significance of Bone-marrow Pattern and Immunophenotypic Score in B-chronic Lymphocytic Leukemia at Diagnosis

Jahic, Azra; Iljazovic, Ermina; Arnautovic-Custovic, Aida; Halilbasic, Alma; Simendic, Vlastimir; Zabic, Aida
June 2011
Medical Archives / Medicinski Arhiv;2011, Vol. 65 Issue 3, p132
Academic Journal
Introduction: The clinical course and outcome of B-CLL is various and so far unpredictible. Defining prognostic parameters potentiating division of patients in groups with favorable and unfavorable prognosis which could help the benefit assessment of early treatment, improve treatment effects, and potentiate treatment modification for each patient. Aim: To analyze the bone-marrow (BM) pattern and immunophenotypic score at diagnosis of B-CLL and determine the correlation of BM pattern with the clinical stage of disease and immunophenotypic score. Methods: A sample of 40 untreated patients with B-CLL was divided into two groups: group with clinical stage Binet A and group with clinical stages Binet B and Binet C. BM patterns were observed as a diffuse, interstitial, nodular and mixed. BM immunophenotyping included CD5, CD23, CD22, and CD20 as an indirect indicator of FMC7. Results: The overall sample mean age was 62.88 years ± 11.10, without significant difference in the age of two compared groups (63.15 ± 10.53 years vs. 62.60 ± 11.50 years) (t = 0.16, df = 38, p = 0.88). Proportion of men was significantly higher in stages Binet B and C (12/20) compared to stage Binet A (5/20) (Z=2.24, p=0.025). The percentage of women was higher than men in Binet A stage (75% vs. 25%). The BM patterns in Binet A stage were observed as follows: mixed 50% (10/20), interstitial 30% (6/20), nodular 15% (3/20) and diffuse 5% (1/20). he BM patterns in Binet B and C stages were observed as follows: diffuse 50% (10/20), mixed 40% (8/20), interstitial 5% (1/20) and nodular 5% (1/20). Clinical stage and the BM patterns were significantly associated (c2=8.02, p=0,005). The chance for non-diffuse patterns was 19 times higher in stage Binet A compared to stages Binet B and C, respectively, analyzing 95% CI at least 2 times higher (95% CI: 2.02-866.6). Immunophenotypic score in total sample was observed as follows: score 4: 5% (2/40), score 3: 72.5% (29/40), score 2: 20% (8/40) and score 1: 2,5% (1/40). Immunophenotypic score 3 and > 3 had 77.5% of patients (31/40), but there was no significant association between the immunophenotypic score and the BM patterns (c2=0.76, p=0.38). Conclusions: Diffuse BM pattern was significantly associated with the clinical stages Binet B and C, compared to non-diffuse BM patterns which were significantly associated with the clinical stage Binet A. Diffuse BM pattern represent the parameter of progressive disease compared to the non-diffuse BM patterns which are more often represented in stable disease. Immunophenotypic score improves diagnostic accuracy of B-CLL, but should not be used as a prognostic parameter of B -CLL


Related Articles

  • CELLULAR IMMUNOPHENOTYPING IN THE DIAGNOSIS OF CHRONIC LYMPHOID LEUKEMIAS. Sánchez, M.; Marsán, V.; Socarrás, B.; Rivero, R. A.; Martínez, M.; Hernández, P.; Macías, C.; Del Valle, L. O. // Revista VacciMonitor (Vacunología y Temas Afines);Oct2002, Vol. 11 Issue 4, p1 

    In order to know the relative frequency of Chronic Lymphoid Leukemia (CLL) subtypes as well as the most frequent cellular antigen expression in patients with CLL diagnosis during the last 10 years, two laboratory methods: an Immunocytochemical Ultramicro method (ICUM) and the Alkaline...

  • CHALLENGING CASES. Engstrom Jr., Robert E. // Ocular Surgery News;7/25/2014 Supplement, p10 

    The article presents several case studies of patients suffering from ophthalmic disorders treated with ocriplasmin injection. The topics include medical aspects of a 71-year-old female patient presented with vitreomacular adhesion, a 73-year-old female patient with vitreomacular traction, and an...

  • The significance of tumour cell immunophenotype in myeloma and its impact on clinical outcome. N-T Ngo // Journal of Clinical Pathology;Nov2009, Vol. 62 Issue 11, p1009 

    BACKGROUND AND AIMS: Antigen expression of multiple myeloma (MM) cells is heterogeneous. We have investigated the clinical impact of expression of some of the commonly used immunohistochemical markers in the diagnostic work-up of bone marrow trephine biopsy (BMTB) samples in MM. PATIENTS AND...

  • In Reply. SAMUELS, NOAH; MAIMON, YAIR; SIEGELMANN-DANIELI, NAVA // Oncologist;May2012, Vol. 17 Issue 5, following p742 

    A response by Noah Samuels, Yair Maimon, and Nava Siegelmann-Danieli to a letter to the editor regarding their research which examines the botanical compound mixture of LCS101 on chemotherapy-induced hematological toxicities in breast cancer patients.

  • Improving but Inferior Survival in Patients with Chronic Lymphocytic Leukemia in Taiwan: A Population-Based Study, 1990–2004. Wu, Shang-Ju; Chiang, Chun-Ju; Lin, Chien-Ting; Tien, Hwei-Fang; Lai, Mei-Shu // PLoS ONE;Apr2013, Vol. 8 Issue 4, p1 

    Background: Chronic lymphocytic leukemia (CLL) is much less prevalent in Asian countries. Whether there are differences in survival outcomes between the East and West, however, remain unclear. Methods: The survival data for CLL patients identified in the Taiwan Cancer Registry database between...

  • Femoral condyle insufficiency fractures: associated clinical and morphological findings and impact on outcome. Plett, Sara; Hackney, Lauren; Heilmeier, Ursula; Nardo, Lorenzo; Yu, Aihong; Zhang, Chiyuan; Link, Thomas; Plett, Sara K; Hackney, Lauren A; Zhang, Chiyuan A; Link, Thomas M // Skeletal Radiology;Dec2015, Vol. 44 Issue 12, p1785 

    Objective: To determine the characteristics of femoral condyle insufficiency fracture (FCIF) lesions and their relative associations with the risk of clinical progression.Materials and Methods: This HIPAA-compliant retrospective study was approved by our Institutional...

  • Síndrome hemofagocítica devido a sarcoidose pulmonar. Bártholo, Thiago Prudente; Pugliese, José Gustavo; Mafort, Thiago Thomaz; Lemos da Silva, Vinicius; Henrique da Costa, Cláudia; Rufino, Rogério // Jornal Brasileiro de Pneumologia;sep/oct2012, Vol. 38 Issue 5, p666 

    Although hemophagocytic syndrome is a rare clinical condition, it is associated with high mortality and the number of cases described in the literature has progressively increased. The diagnosis of hemophagocytic syndrome is made on the basis of a finding of hemophagocytosis. Sarcoidosis is a...

  • Results from Alzheimer's Vaccine Trial Announced.  // Journal of Gerontological Nursing;May2011, Vol. 37 Issue 5, p29 

    The article reports on the results of a clinical trial of a vaccine for Alzheimer's disease called AD02 which found that the vaccine may be effective in treating the disease.

  • Transepithelial cross-linking may be less efficient in treating progressive keratoconus.  // Ocular Surgery News;9/25/2012, Vol. 30 Issue 18, p23 

    The article discusses the results of a study, published in the "Journal of Cataract & Refractive Surgery," which examined the effectiveness of using transepithelial collagen cross-linking in treating progressive keratoconus.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics