TITLE

Technical and financial evaluation of assays for progesterone in canine practice in the UK

AUTHOR(S)
Moxon, R.; Copley, D.; England, G. C. W.
PUB. DATE
October 2010
SOURCE
Veterinary Record: Journal of the British Veterinary Association;Oct2010, Vol. 167 Issue 14, p528
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The concentration of progesterone was measured in 60 plasma samples from bitches at various stages of the oestrous cycle, using commercially available quantitative and semi-quantitative ELISA test kits, as well as by two commercial laboratories undertaking radioimmunoassay (RIA). The RIA, which was assumed to be the ‘gold standard’ in terms of reliability and accuracy, was the most expensive method when analysing more than one sample per week, and had the longest delay in obtaining results, but had minimal requirements for practice staff time. When compared with the RIA, the quantitative ELISA had a strong positive correlation (r=0.97, P<0.05) and a sensitivity and specificity of 70.6 per cent and 100.0 per cent, respectively, and positive and negative predictive values of 100.0 per cent and 71.0 per cent, respectively, with an overall accuracy of 90.0 per cent. This method was the least expensive when analysing five or more samples per week, but had longer turnaround times than that of the semi-quantitative ELISA and required more staff time. When compared with the RIA, the semi-quantitative ELISA had a sensitivity and specificity of 100.0 per cent and 95.5 per cent, respectively, and positive and negative predictive values of 73.9 per cent and 77.8 per cent, respectively, with an overall accuracy of 89.2 per cent. This method was more expensive than the quantitative ELISA when analysing five or more samples per week, but had the shortest turnaround time and low requirements in terms of staff time.
ACCESSION #
66322205

 

Related Articles

  • Proliferation and apoptosis in normal bitch mammary tissues in relation to progesterone level. Manee-in, Sukanya; Srisuwatanasagul, Sayamon // Comparative Clinical Pathology;Oct2012, Vol. 21 Issue 5, p615 

    The objective of this study was to investigate the proliferating activity and apoptosis in canine normal mammary tissues in different cycle stages. Mammary tissues were collected from five beagle bitches at six different estrous stages: anestrus, proestrus, estrus, early diestrus, mid diestrus,...

  • PROGESTERONE ASSAY.  // Materials Management in Health Care;Mar2009, Vol. 18 Issue 3, p6 

    The article evaluates the AU3000i progesterone assay from Olympus America Inc.

  • Help the Canine Mind. Moran, Peggy // Dog World;Dec2000, Vol. 85 Issue 12, p130 

    Advocates the practice of canine socialization.

  • Preclinical Evaluation of a Novel Artificial Tear for Dry Eye. Shovlin, Joseph P.; DeAngelis, Therese // Review of Optometry;5/15/2004 Supplement, p37 

    Develops a unique battery of in vitro and in vivo tests for evaluating the ocular safety of a novel artificial tear solution. Evaluation of cytotoxicity in an exaggerated neutral red release assay; Use of Madin-Darby canine kidney cells of an agar overlay assay; Imaging of corneal epithelial...

  • A Case of Progesterone-Induced Anaphylaxis, Cyclic Urticaria/Angioedema, and Autoimmune Dermatitis. Bernstein, I. Leonard; Bernstein, David I.; Lummus, Zana L.; Bernstein, Jonathan A. // Journal of Women's Health (15409996);Apr2011, Vol. 20 Issue 4, p643 

    Objective: Women have exhibited anaphylaxis, urticaria/angioedema, and autoimmune progesterone dermatitis (APD) coinciding with the progesterone premenstrual rise. We report a detailed immunological evaluation of such a woman responsive to a gonadotropin hormone-releasing agonist (GHRA)....

  • The Technical Quiz.  // Money Marketing;5/24/2012, p48 

    A quiz about financial, retirement and tax planning from www.moneymarketing.co.uk/technical is presented.

  • 1000 canine donors - now Pet Blood Bank seeks cats too.  // Veterinary Record: Journal of the British Veterinary Association;12/6/2008, Vol. 163 Issue 23, p671 

    The article focuses on the canine blood donation service of the Pet Blood Bank UK (PBBuk) and its plan to launch a feline blood donation in Great Britain. PBBuk is blood bank that collects blood from qualified canine donor and send the plasma to veterinary practice and hospitals. It cites that...

  • EVALUATION OF R&D PROJECTS IN PHARMACEUTICAL INDUSTRY: DO PHARMACEUTICAL COMPANIES ADEQUATELY ACCOUNT FOR RISK AND FLEXIBILITY? Vujanović, Nikola; Lončar, Ivana // Proceedings of the International May Conference on Strategic Man;May2016, p302 

    The aim of the paper is to explore the level of project evaluation sophistication among pharmaceutical companies. Literature review and the empirical study in the UK pharmaceutical company revealed very low confidence in financial analysis when making important project decisions. Additionally,...

  • The skeptic.  // Redbook;Sep2009, Vol. 213 Issue 3, p34 

    The article evaluates the Jog a Dog , a canine motorized treadmill.

  • Canine Good Citizen Close-Up. Carpenter, Sherry // Dog World;Jul2001, Vol. 86 Issue 7, p54 

    Focuses on the changes to the Canine Good Citizen evaluation guidelines.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics