Prevalence of Coxiella burnetii infection in Dutch dairy herds based on testing bulk tank milk and individual samples by PCR and ELISA

Muskens, J.; van Engelen, E.; van Maanen, C.; Bartels, C.; Lam, T. J. G. M.
January 2011
Veterinary Record: Journal of the British Veterinary Association;Jan2011, Vol. 168 Issue 3, p79
Academic Journal
A study using an ELISA and a real-time PCR assay based on the detection of the repetitive transposon-like gene of revealed that infection with the bacterium was widespread among Dutch dairy herds, with antibodies detected in bulk tank milk (BTM) from 268 of 341 herds (78.6 per cent) and bacterial DNA detected in 193 of 341 herds (56.6 per cent). The BTM samples were taken in November and December 2007. Serological and molecular studies in young and adult cattle selected from 100 herds showed that antibodies were present in the blood of 470 of 2936 (16.0 per cent) lactating cows but only in 19 of 1831 (1.0 per cent) young animals. Bacterial DNA was detected in the milk of 254 of 2925 (8.7 per cent) lactating cows; bacterial DNA was not detected in any of the faecal samples obtained from youngstock. The blood and milk samples were taken from the cattle in the period January to April 2008.


Related Articles

  • Coxiella burnetii in Bulk Tank Milk Samples,. Sung Guk Kim; Eun Hee Kim; Lafferty, Caroline J.; Dubovi, Edward // Emerging Infectious Diseases;Apr2005, Vol. 11 Issue 4, p619 

    Dairy cattle are a primary reservoir of Coxiella burnetii, which causes Q fever. However, no recent nationwide studies have assessed the prevalence and risks of Q fever in dairy cattle. We report >94% prevalence in samples of bulk tank milk from U.S. dairy herds tested during the past years.

  • Prevalence of Coxiella burnetii antibodies in Danish dairy herds. Agger, Jens F.; Christoffersen, Anna-Bodil; Rattenborg, Erik; Nielsen, Jørgen; Agerholm, Jørgen S. // Acta Veterinaria Scandinavica;2010, Vol. 52, p1 

    During recent years in Denmark higher rates of antibodies to Coxiella burnetii have been detected in animals and humans than previously reported. A study based on bulk tank milk samples from 100 randomly selected dairy herds was performed to estimate the prevalence and geographical distribution...

  • Increasing prevalence of Coxiella burnetii seropositive Danish dairy cattle herds. Agger, Jens Frederik; Paul, Suman // Acta Veterinaria Scandinavica;2014, Vol. 56 Issue 1, p46 

    A study based on bulk tank milk samples from 120 randomly selected dairy cattle herds was conducted to estimate the prevalence of Coxiella burnetii seropositive dairy herds, to describe the geographical distribution, and to identify risk factors. Using the CHEKIT Q-fever Antibody ELISA Test Kit...

  • Estimated herd prevalence and sequence types of Coxiella burnetii in bulk tank milk samples from commercial dairies in Indiana. Bauer, Amy E.; Olivas, Sonora; Cooper, Maria; Hornstra, Heidie; Keim, Paul; Pearson, Talima; Johnson, April J. // BMC Veterinary Research;Aug2015, Vol. 11 Issue 1, p1 

    Background: Coxiella burnetii is the etiologic agent of Q fever, a zoonotic disease causing influenza-like illness, pregnancy loss, cardiovascular disease and chronic fatigue syndrome in people. C. burnetii is considered to be enzootic in ruminants, but clinical signs of infection do not always...

  • Prevalence of Coxiella burnetii antibodies in Portuguese dairy cattle herds. Pimenta, Luís; Alegria, Nuno; Anastácio, Sofia; Sidi-Boumedine, Karim; Silva, Gabriela; Rabiço, Ângela; Simões, João // Tropical Animal Health & Production;Jan2015, Vol. 47 Issue 1, p227 

    Q fever is an important zoonotic disease which has been recently diagnosed, mainly in sheep and goats, in Portugal. The aim of the present study was to determine the prevalence of bovine Coxiella burnetii antibodies in dairy farms from the northwest of Portugal. Bulk tank milk samples were...

  • Coxiella burnetii in bulk tank milk samples from dairy goat and dairy sheep farms in the Netherlands in 2008. den Brom, R. van; van Engelen, E.; Luttikholt, S.; Moll, L.; van Maanen, K.; Vellema, P. // Veterinary Record: Journal of the British Veterinary Association;3/24/2012, Vol. 170 Issue 12, p310 

    The article presents a study on the prevalence of Q fever among samples of dairy goat and sheep bulk tank milk (BTM) in the Netherlands. The study used various methods such as real-time polymerase chain reaction (RT-PCT), enzyme-linked immunosorbent assay (ELISA) and immunohistorchemistry (IHC)....

  • Progression of Coxiella burnetii infection after implementing a two-year vaccination program in a naturally infected dairy cattle herd. Piñero, Alvaro; Barandika, Jesús F.; Hurtado, Ana; García-Pérez, Ana L. // Acta Veterinaria Scandinavica;2014, Vol. 56 Issue 1, p1 

    Background The high prevalence of Coxiella burnetii infection in dairy cattle herds recently reported and the long survival time of the bacterium in the environment pose a risk to human and animal health that calls for the implementation of control measures at herd level. This study presents the...

  • Evaluation of Two PCR Tests for Coxiella burnetii Detection in Dairy Cattle Farms Using Latent Class Analysis. Nusinovici, Simon; Madouasse, Aurélien; Hoch, Thierry; Guatteo, Raphaël; Beaudeau, François // PLoS ONE;12/16/2015, Vol. 10 Issue 12, p1 

    Different tests performed on bulk tank milk samples (BTM) are available to determine the C. burnetii status of herds. However, these tests, which are based on the detection of either antibodies directed against C. burnetii (ELISA) or bacterial DNA (PCR), have limitations. A currently...

  • Prevalence of Coxiella burnetii in bovine bulk milk samples in southern Iran. Kargar, Mohammad; Rashidi, Afsaneh; Doosti, Abbas; Ghorbani-Dalini, Sadegh; Najafi, Akram // Comparative Clinical Pathology;May2013, Vol. 22 Issue 3, p331 

    One hundred (100) bovine bulk milk samples were tested for the presence of Coxiella burnetii and the detection of associated risk factors. Of the tested samples, 11% tested positive for C. burnetii. There was no correlation between the presence of C. burnetii in bulk milk samples and the kind of...

  • Occurrence of Coxiella burnetii in bulk tank milk from northwestern Italy. Vicari, N.; Faccini, S.; Ricchi, M.; Garbarino, C.; Decastelli, L.; Boldini, M.; Rosignoli, C.; Dalmasso, A.; Bronzo, V.; Fabbi, M. // Veterinary Record: Journal of the British Veterinary Association;6/29/2013, Vol. 172 Issue 26, p1 

    The article discusses the occurrence of Coxiella burnetii infection in dairy cattle herds which found in bulk tank of milk from northwestern Italy. It elaborates the common clinical signs in domestic ruminants including infertility, non-viable neonates and abortion. Also investigated the...


Read the Article


Sign out of this library

Other Topics