Cytokine expression in normal, atopic, and asthmatic subjects using the combination of sputum induction and the polymerase chain reaction

Gelder, C M; Thomas, P S; Yates, D H; Adcock, I M; Morrison, J F; Barnes, P J
October 1995
Thorax;Oct1995, Vol. 50 Issue 10, p1033
Academic Journal
No abstract available.


Related Articles

  • Inflammatory response in induced sputum mononuclear cells from patients with acute exacerbation of asthma. Hamzaoui, A.; Brahim, M. Ben; Zhioua, A.; Ayed, Kh.; Hamzaoui, K. // Mediators of Inflammation;Aug2000, Vol. 9 Issue 3/4, p147 

    Examination of sputum provides a direct method to investigate airway inflam mation non-invasively in particular Th1 (IL-2, IFN-γ) and Th2 (IL-4, IL-10) cytokine production. IL-2, IL-4, IL-10 and IFN-γ cytokine were studied in induced sputum mononuclear cells of asthmatic patients. Sputum...

  • Expression of Cytokine mRNA in Bronchoalveolar Lavage Cells from Atopic Asthmatics before Late Antigen-Induced Reaction. Miadonna, A.; Gibelli, S.; Lorini, M.; Tedeschi, A.; Oddera, S.; Rossi, G.A.; Crimi, E. // Lung;1997, Vol. 175 Issue 3, p195 

    Recently, much attention has been given to the possible role of lymphocytes and their soluble products in causing and maintaining allergic inflammation. The aim of this study was to assess the production of mRNAs for interleukins (IL) in bronchoalveolar lavage (BAL) cells obtained from...

  • The usefulness of competitive PCR: airway gene expression of IL-5, IL-4, IL-4, IL-4δ2, IL-2, and IFNγ in asthma. Glare, E. M.; Divjak, M.; Bailey, M. J.; Walters, E. H. // Thorax;Jul2001, Vol. 56 Issue 7, p541 

    Background--Asthma has been described as an eosinophilic bronchitis driven by interleukin (IL)-4 and IL-5. The quantification of cytokine mRNA levels in airway samples has been confounded by housekeeping gene expression which differs between and within asthmatics and controls. Methods--The...

  • Sputum IgE and Cytokines in Asthma: Relationship with Sputum Cellular Profile. Manise, Maïté; Holtappels, Gabriele; Van Crombruggen, Koen; Schleich, Florence; Bachert, Claus; Louis, Renaud // PLoS ONE;Mar2013, Vol. 8 Issue 3, p1 

    Background: Local IgE production may play a role in asthma pathogenesis. The aim of the study was to assess sputum total IgE and cytokines in asthmatics according to sputum cellular phenotype. Methods: We studied 122 subjects including 22 non atopic healthy subjects, 41 eosinophilic...

  • Epidermal Cytokines IL-1β, TNF-α, and IL-12 in Patients with Atopic Dermatitis: Response to Application of House Dust Mite Antigens1. Junghans, Volker; Gutgesell, Carsten; Jung, Thomas; Neumann, Christine // Journal of Investigative Dermatology;Dec98, Vol. 111 Issue 6, p1184 

    Epidermal cytokines such as interleukin (IL)-1β, tumor necrosis factor-α, and IL-12 have been described to play a crucial role in the induction and elicitation phase of allergic contact dermatitis upon exposure to haptens. In this study we asked whether these cytokines may also play a role...

  • Endogenous Antimicrobial Peptides and Skin Infections in Atopic Dermatitis. Ong, Peck Y.; Ohtake, Takaaki; Brandt, Corinne; Strickland, Ian; Boguniewicz, Mark; Ganz, Tomas; Gallo, Richard L.; Leung, Donald Y.M. // New England Journal of Medicine;10/10/2002, Vol. 347 Issue 15, p1151 

    Background: The innate immune system of human skin contains antimicrobial peptides known as cathelicidins (LL-37) and β-defensins. In normal skin these peptides are negligible, but they accumulate in skin affected by inflammatory diseases such as psoriasis. We compared the levels of...

  • Dysregulation of cytokine expression in tubulointerstitial nephritis associated with murine malaria. Li Rui-Mei; Kara, A.U.; Sinniah, Raja // Kidney International;Apr1998, Vol. 53 Issue 4, p845 

    Examines the dysregulation of cytokine expression in tubulointerstitial nephritis associated with murine malaria. Assessment of renal disfunction; Measurements of the circulating cytokine; Use of the reverse transcription-polymerase chain reaction.

  • Evaluation of airway inflammation by quantitative Th1 /Th2 cytokine mRNA measurement in sputum of asthma patients. Truyen, E.; Coteur, I.; Dilissen, E.; Overbergh, I.; Dupont, L. J.; Ceuppens, J. L.; Bullens, D. M. A. // Thorax;Mar2006, Vol. 61 Issue 3, p202 

    Background: Asthma is a chronic inflammatory disorder of the airways driven by I cell activation. Th2 cells and their cytokines are thought to play a role in the pathophysiology of allergic as well as non-allergic asthma. Methods: Airway cells were obtained by sputum induction from 15 healthy...

  • The Cytokine Network in Lesional and Lesion-Free Psoriatic Skin Is Characterized by a T-Helper Type 1 Cell-Mediated Response. Uyemura, Koichi; Yamamura, Masahiro; Fivenson, David F.; Modlin, Robert L.; Nickoloff, Brian J. // Journal of Investigative Dermatology;Nov93, Vol. 101 Issue 5, p701 

    As a psoriatic lesion develops at sites of previously uninvolved skin, cytokines and their subsequent induction of various adhesion molecules may play important pathophysiologic roles. To further define the cytokine network in psoriasis, biopsies were obtained from both lesional skin and...

  • TLR3, TLR4 and TLRs7–9 Induced Interferons Are Not Impaired in Airway and Blood Cells in Well Controlled Asthma. Sykes, Annemarie; Edwards, Michael R.; Macintyre, Jonathan; del Rosario, Ajerico; Gielen, Vera; Haas, Jennifer; Kon, Onn Min; McHale, Mark; Johnston, Sebastian L. // PLoS ONE;Jun2013, Vol. 8 Issue 6, p1 

    Defective Rhinovirus induced interferon-β and interferon-λ production has been reported in bronchial epithelial cells from asthmatics but the mechanisms of defective interferon induction in asthma are unknown. Virus infection can induce interferon through Toll like Receptors (TLR)3, TLR7...


Read the Article


Sign out of this library

Other Topics