TITLE

Comparison of Responses Elicited by Immunization with a Legionella Species Common Lipoprotein Delivered as Naked DNA or Recombinant Protein

AUTHOR(S)
Yoon, Won Suck; Park, Seol Hee; Park, Yong Keun; Park, Seung Chul; Sin, Jeong Im; Kim, Min Ja
PUB. DATE
February 2002
SOURCE
DNA & Cell Biology;Feb2002, Vol. 21 Issue 2, p99
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
To evaluate the peptidoglycan-associated lipoprotein (PAL) antigen of Legionella pneumophila as a vaccine candidate, mice were immunized intramuscularly with pcDNA3-PAL and intraperitoneally with recombinant PAL (t-rPAL), which were compared for their ability to induce PAL-specific immune responses. The t-rPAL protein induced PAL-specific IgG antibody production significantly more than did pcDNA3-PAL. The IgG[sub 2a] and IgG[sub 1] production was predominant after pcDNA3-PAL and t-rPAL administration, respectively. In particular, pcDNA3-PAL induced much higher PAL-specific cytotoxic T-lymphocyte responses than did t-rPAL. Furthermore, in vivo, CD19[sup +] B-cell populations were dramatically increased by t-rPAL vaccination, suggesting a B-cell immunomodulatory activity of the lipoprotein. The PAL antigen was also conserved among Legionella species, as determined by PCR and immunoblot analyses. These results support a potential use of the t-rPAL protein and in particular DNA vaccines against Legionella infections.
ACCESSION #
6574031

 

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