Discrepancies between a new highly sensitive Toxoplasma gondii ELISA assay and other reagents: interest of Toxo IgG Western blot

Lesl�, F.; Touafek, F.; Fekkar, A.; Mazier, D.; Paris, L.
October 2011
European Journal of Clinical Microbiology & Infectious Diseases;Oct2011, Vol. 30 Issue 10, p1207
Academic Journal
Immunodiagnostic assays are commonly used to screen for maternal toxoplasmic seroconversion during pregnancy. The introduction to the market of a new highly sensitive IgG assay, the Elecsys Toxo IgG test, has resulted in discrepancy issues with other immunoassays because of a lack of standardisation. Western blot appears to be a good alternative gold standard to the dye test, as the latter is not routinely available. For the present prospective study, we compared the analytical performances of two immunoassays, Elecsys Toxo IgG (Roche Diagnostics) and Platelia Toxo IgG (Bio-Rad, Marnes la Coquette, France), to Toxo II IgG Western blot (LDBio, Lyon, France) using 231 consecutive sera with low or equivocal IgG titres. Of these 231 sera, 213 presented discrepancies, which showed the importance of a confirmation test. Of the Elecsys Toxo IgG-positive results, 100% were confirmed by the Western blot with a positive threshold of 30 IU/ml for Elecsys; in the equivocal area (1-30 IU/ml), Western blot is negative in 54% of cases. Our results suggest that the lower diagnostic cut-off of Platelia Toxo IgG should be further reduced. Our study indirectly confirms that monitoring, especially for pregnant women, must be done in the same laboratory using the same technique. The ability to diagnose very early seroconversion using Western blot merits further study.


Related Articles

  • IgG AVIDITY WESTERN BLOT USING Toxoplasma gondii rGRA-7 CLONED FROM NUCLEOTIDES 39-711 FOR SERODIAGNOSIS OF ACUTE TOXOPLASMOSIS. DESHPANDE, Poonam S.; KOTRESHA, Dupadahalli; NOORDIN, Rahmah; YUNUS, Muhammad Hafiznur; SAADATNIA, Geita; GOLKAR, Majid; OSMAN, Sabariah; Abdul KARIM, Izzati Zahidah; GHAFFARIFAR, Fatemeh // Revista do Instituto de Medicina Tropical de Sao Paulo;Mar/Apr2013, Vol. 55 Issue 2, p79 

    Toxoplasmosis is an important cause of congenital infection. The present study was performed to evaluate the usefulness of recombinant (r) GRA-7 cloned from nucleotides (n) 39-711 in discriminating between acute and chronic toxoplasmosis. First, commercial IgM, IgG and IgG avidity ELISAs were...

  • Diurnal variation and age-related changes of bone turnover markers in female Göttingen minipigs. H. Tsutsumi; K. Katagiri; M. Morimoto; T. Nasu; M. Tanigawa; K. Mamba // Laboratory Animals;Oct2004, Vol. 38 Issue 4, p439 

    We investigated diurnal variation and age-related changes in bone turnover markers in female Göttingen minipigs. Ten females, 6–9 months of age, were used for confirmation of diurnal variation. Blood was collected at 3 h intervals for 24 h, and bone-specific alkaline phosphatase and...

  • Effect of some physico-chemical conditions on an immunoassay for viable Escherichia coli. Jurkevica, Agnese; Halsall, H. Brian; Seliskar, Carl J.; Heineman, William R. // Analytical & Bioanalytical Chemistry;Aug2010, Vol. 397 Issue 7, p3133 

    Viable Escherichia coli can be detected by an immunoassay in which live bacteria captured on antibody-coated paramagnetic beads are induced to synthesize the enzyme β-galactosidase, which catalyzes the hydrolysis of the slightly fluorescent substrate 4-methyl umbelliferyl-β- d-galactoside...

  • Evaluation of Diagnostic Value in Using a Panel of Multiple Tumor-Associated Antigens for Immunodiagnosis of Cancer. Peng Wang; Chunhua Song; Weihong Xie; Hua Ye; Kaijuan Wang; Liping Dai; Yi Zhang; Jianying Zhang // Journal of Immunology Research;2014, p1 

    To determine whether a panel of multiple tumor-associated antigens (TAAs) would enhance antibody detection, the diagnostic value of autoantibodies to a panel of multiple TAAs in cancer has been evaluated. The TAAs used in this study was composed of eight TAAs including Imp1, p62, Koc, p53,...

  • Multiple immunoassay systems are negatively interfered by circulating cardiac troponin I autoantibodies. Tang, Gusheng; Wu, Yu; Zhao, Weiguo; Shen, Qian // Clinical & Experimental Medicine;Mar2012, Vol. 12 Issue 1, p47 

    Circulating cardiac troponin I (cTnI) autoantibodies have recently been detected in more and more patients with myocardial injury. In the present study, a total of 121 patients with acute myocardial infarction (AMI) were screened for cTnI autoantibodies using an indirect ELISA. Positive results...

  • A novel monoclonal antibody immunoassay for the detection of human serum hepcidin. Schwarz, Peggy; Strnad, Pavel; Figura, Guido; Janetzko, Alfred; Krayenbühl, Pierre; Adler, Guido; Kulaksiz, Hasan // Journal of Gastroenterology;May2011, Vol. 46 Issue 5, p648 

    Background: Hepcidin is a liver-derived peptide hormone regulating iron metabolism. Changes in the expression of hepcidin are known to be the key pathogenic factors in hereditary hemochromatosis and are associated with infection and inflammation. To better understand the hormone's function in...

  • Chapter 12: Serology. Van Regenmortel, Marc H. V.; Khan, Jawaid A.; Dijkstra, Jeanne // Handbook of Plant Virology;2006, p145 

    The article focuses on immunoassays or immunochemical techniques. An enzyme-linked immunosorbent assay is a solid-phase immunoassay on a plastic surface and the reaction is detected by means of enzyme-labeled antibodies. In plant virology, various immunoblotting assays are commonly used, such as...

  • Comparison of a rapid immunoassay for antibodies to the C6 antigen with conventional tests for antibodies to Borrelia burgdorferi in dogs in Europe. Gerber, B.; Haug, K.; Eichenberger, S.; Reusch, C. E.; Wittenbrink, M. M. // Veterinary Record: Journal of the British Veterinary Association;11/14/2009, Vol. 165 Issue 20, p594 

    A commercial immunoassay for antibodies to the C6 antigen of Borrelia burgdorferi was evaluated against an IgG in-house ELISA in combination with a Western blot assay to examine 104 samples of serum from 53 healthy Bernese mountain dogs, which were suspected to have a breed predisposition to...

  • Development of a new immunoassay for the accurate determination of anti-infliximab antibodies in inflammatory bowel disease. Imaeda, Hirotsugu; Andoh, Akira; Fujiyama, Yoshihide // Journal of Gastroenterology;Feb2012, Vol. 47 Issue 2, p136 

    Background: The formation of antibodies to infliximab (ATIs) is closely associated with the loss of response to infliximab in patients with inflammatory bowel disease (IBD). We evaluated the clinical utility of a novel method to measure serum ATI levels in the presence of infliximab. Methods:...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics