Metallo beta lactamase producing Pseudomonas aeruginosa and Acinetobacter baumannii

John, S; R, Balagurunathan
July 2011
Indian Journal of Medical Microbiology;Jul2011, Vol. 29 Issue 3, p302
Academic Journal
This study aims in identifying MBLs particularly Zn requiring Molecular Class B enzymes produced by Pseudomonas aeruginosa and Acinetobacter baumannii .The resistance by these organisms are in a rise against all antibiotics including carbapenems and no prescribed CLSI guidelines is available for detecting them. Clinical isolates antibiotic susceptibility was determined by number of phenotypic tests by addition of 50mM of 10 μl zinc as cofactor for metallo beta lactamase production along with 0.5M ETDA of 5μl (930 μg per disk) plain disks. Increase in zone size of the meropenem -EDTA disk compared to the meropenem disk without EDTA was recorded positive. For Zn requiring MBLs zone towards both disks of EDTA and Zn along with meropenem is detected by DDST.


Related Articles

  • Doripenem. Paterson, David L.; DePestel, Daryl D. // Clinical Infectious Diseases;7/15/2009, Vol. 49 Issue 2, p291 

    Carbapenems play a significant role in the current antibiotic armamentarium. Doripenem is the newest carbapenem to be commercially released. Its antimicrobial spectrum more closely resembles those of meropenem and imipenem than that of ertapenem. Thus, it has significant in vitro activity...

  • Prevalence and molecular characterisation of metallo-beta-lactamase producing strains of imipenem-resistant Pseudomonas aeruginosa in Turkey. Yilmaz, N. Ozkalay; Agus, N.; Bozcal, E.; Uzel, A. // Indian Journal of Medical Microbiology;Jul-Sep2014, Vol. 32 Issue 3, p349 

    The article discusses the study conducted for identifying the imipenem (IMP) resistant strains of Pseudomonas aeruginosa from clinical specimens of inpatients and investigating the presence of metallo beta lactamases (MBL)-producing strains by phenotypic and genotypic methods. Topics discussed...

  • Novel derivatives of polymyxins. Vaara, Martti // Journal of Antimicrobial Chemotherapy (JAC);Jun2013, Vol. 68 Issue 6, p1213 

    Polymyxin B and colistin (polymyxin E) are bactericidal pentacationic lipopeptides that act specifically on Gram-negative bacteria, first by disrupting their outermost permeability barrier, the outer membrane (OM), and then damaging the cytoplasmic membrane. Both were discovered in the mid-1950s...

  • Complex traits: Extreme phenotypes assist association studies. Burgess, Darren J. // Nature Reviews Genetics;Sep2012, Vol. 13 Issue 9, p597 

    The article discusses research on the identification of complex trait genes using exome sequencing, which references the study "Exome sequencing of extreme phenotypes identifies DCTN4 as a modifier of chronic Pseudomonas aeruginosa infection in cycstic fibrosis," by M. J. Emond and colleagues in...

  • Detection of Pseudomonas aeruginosa isolates producing VEB-type extended-spectrum {beta}-lactamases in the United Kingdom. Neil Woodford; Jiancheng Zhang; Mary E. Kaufmann; Susannah Yarde; Maria del Mar Tomas; Camelia Faris; Madhur S. Vardhan; Susan Dawson; Stephen L. Cotterill; David M. Livermore // Journal of Antimicrobial Chemotherapy (JAC);Dec2008, Vol. 62 Issue 6, p1265 

    : Objectives The aim of this study was to investigate the presence of VEB enzymes among Pseudomonas spp. referred to the UKs national reference laboratory and with phenotypic evidence of extended-spectrum β-lactamase (ESBL) production. : Methods Antibiograms were analysed for Pseudomonas...

  • Characterization of a novel Zn2+-dependent intrinsic imipenemase from Pseudomonas aeruginosa. Fajardo, Alicia; Hernando-Amado, Sara; Oliver, Antonio; Ball, Geneviève; Filloux, Alain; Martinez, Jose L. // Journal of Antimicrobial Chemotherapy (JAC);Nov2014, Vol. 69 Issue 11, p2972 

    Objectives Previous work showed that PA5542 inactivation increases Pseudomonas aeruginosa 59.20 susceptibility to carbapenems. The objective of the current study was to purify PA5542, to determine its role in carbapenem resistance and to analyse the kinetic constants of this putative new...

  • Dissemination of Pseudomonas aeruginosa producing blaIMP-1 and blaVIM-1 in Qazvin and Alborz educational hospitals, Iran. Peymani, Amir; Farivar, Taghi Naserpour; Ghanbarlou, Mahdi Mohammadi; Najafipour, Reza // Iranian Journal of Microbiology;Dec2015, Vol. 7 Issue 6, p302 

    Background and Objectives: Pseudomonas aeruginosa is a frequent opportunistic pathogen in health care associated infections that is highly resistant to the majority of β-lactams. The aims of this study were to access the antimicrobial susceptibility pattern of P. aeruginosa isolated from...

  • First report of the emergence of New Delhi metallo-β-lactamase-1 producing Acinetobacter junii in Nanjing, China. W. Q. Zhou; Z. F. Zhang; H. Shen; M. Z. Ning; X. J. Xu; X. L. Cao; K. Zhang // Indian Journal of Medical Microbiology;Apr2013, Vol. 31 Issue 2, p206 

    A letter to the editor is presented related to a case study of a 77-year-old male patient Nanjing city of Jiangsu province, China regarding the emergence of New Delhi metallo-beta-actamase-1 enzyme that can produce Acinetobacter junii.

  • The Prospective Evaluation of Four Convenient Methods for Detecting MBLs in the Clinical Isolates. PRAKASH, MRIDULA RAJ; VEENA, M.; SHARMA, ARCHANA; BASAVARAJ, K. N.; VISWANATH, G. // Journal of Clinical & Diagnostic Research;Sep2012, Vol. 6 Issue 7, p1196 

    Background: The emergence and the spread of metallo-beta-lactamases (MBLs) in non fermenters like Pseudomonas aeruginosa and the Acinetobacter spp has become a therapeutic challenge. In this study, the phenotypic confirmation of the MBL production was done by different methods. Method: The...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics